Functional expression of diverse post-translational peptide-modifying enzymes in Escherichia coli under uniform expression and purification conditions

Autor: Emerson Glassey, Andrew M. King, Daniel A. Anderson, Zhengan Zhang, Christopher A. Voigt

Publikováno v: PLoS ONE, Vol 17, Iss 9 (2022)

RiPPs (ribosomally-synthesized and post-translationally modified peptides) are a class of pharmaceutically-relevant natural products expressed as precursor peptides before being enzymatically processed into their final functional forms. Bioinformatic

Externí odkaz: https://doaj.org/article/376b11b10e8f4653b9fae81ad846d4a9

Selection for constrained peptides that bind to a single target protein

Autor: Daniel A. Anderson, Amanda C. Embree, Thomas Williams, Andrew M. King, David L. Niquille, Christopher A. Voigt, Thomas H. Segall-Shapiro, Zhengan Zhang, Katelin Pratt, Emerson Glassey, D. Benjamin Gordon

Publikováno v: Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)

Peptide secondary metabolites are common in nature and have diverse pharmacologically-relevant functions, from antibiotics to cross-kingdom signaling. Here, we present a method to design large libraries of modified peptides in Escherichia coli and sc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5af1bb33f5e0c1fc38e67c2cb5664b8
http://europepmc.org/articles/PMC8566587

Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease

Autor: Carly R. Muletz-Wolz, Brian Gratwicke, Christopher A. Voigt, Jennifer A N Brophy, Ed Bronikowski, Matthew H. Becker, Alyssa W. Kaganer, Robert C. Fleischer, Elliot Lassiter, Matthew Evans, Emerson Glassey, Blake Klocke, Kevin Barrett, Adam J. Meyer

Publikováno v: ISME Communications. 1

We designed two probiotic treatments to control chytridiomycosis caused by Batrachochytrium dendrobatidis (Bd) on infected Panamanian golden frogs (Atelopus zeteki), a species that is thought to be extinct in the wild due to Bd. The first approach di

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c3f4b1d73a4a27f36c01b9f4408cd2df
https://doi.org/10.1038/s43705-021-00044-w

Escherichia coli 'Marionette' strains with 12 highly optimized small-molecule sensors

Autor: Jing Zhang, Thomas H. Segall-Shapiro, Emerson Glassey, Adam J. Meyer, Christopher A. Voigt

Publikováno v: Nature chemical biology. 15(2)

Cellular processes are carried out by many genes, and their study and optimization requires multiple levers by which they can be independently controlled. The most common method is via a genetically encoded sensor that responds to a small molecule. H

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9988016685d16687d89946da1e585faa
https://pubmed.ncbi.nlm.nih.gov/30478458

DNAplotlib:programmable visualization of genetic designs and associated data

Autor: Casper Enghuus, Christopher A. Voigt, Bryan Bartley, Daniel B. Goodman, Bryan S. Der, D. Benjamin Gordon, Emerson Glassey, Thomas E. Gorochowski

Publikováno v: Der, B, Glassey, E, Bartley, B, Enghuus, C, Goodman, D, Gordon, B, Voigt, C & Gorochowski, T 2017, ' DNAplotlib : programmable visualization of genetic designs and associated data ', ACS Synthetic Biology, vol. 6, no. 7, pp. 1115-1119 . https://doi.org/10.1021/acssynbio.6b00252

DNAplotlib (www.dnaplotlib.org) is a computational toolkit for the programmable visualization of highly customizable, standards-compliant genetic designs. Functions are provided to aid with both visualization tasks and to extract and overlay associat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b077bf12b0cb5c091b6d75dc45bfb12a
https://research-information.bris.ac.uk/ws/files/89328588/DNAplotlib.pdf