Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Elzbieta Bedner"'
Publikováno v:
Cytometry Part A. :10-18
Background Simian Virus 40 (SV40) infection of growth-arrested monkey kidney cells stimulates S phase entry and the continued synthesis of both viral and cellular DNA. Infected cells can attain total DNA contents as high as DNA Index, DI = 5.0–6.0
Autor:
Frank Traganos, Elzbieta Bedner, Xiaoxing Wang, Sophie Chen, Zbigniew Darzynkiewicz, A. Deptala, Tze-chen Hsieh, Q. Ruan
Publikováno v:
Cell Proliferation. 34:293-304
Recent studies on the Chinese herbal medicine PC SPES showed biological activities against prostate cancer in vitro, in vivo and in patients with advanced stages of the disease. In investigating its mode of action, we have isolated a few of the activ
Autor:
David J. Phelps, Piotr Smolewski, Litong Du, Elzbieta Bedner, Zbigniew Darzynkiewicz, Joseph M. Wu, Tze-chen Hsieh
Publikováno v:
Cytometry. 44:73-82
Background The fluorochrome-labeled inhibitors of caspases (FLICA) were recently used as markers of activation of these enzymes in live cells during apoptosis (Bedner et al.: Exp Cell Res 259:308–313, 2000). The aims of this study were to (a) explo
Publikováno v:
Experimental Cell Research. 260:248-256
Apoptosis is characterized by a complex and remarkably ordered choreography of events consisting of the preparatory and execution steps that all culminate in disposal of the cell remnants. The disposal occurs in a manner that is the least destructive
Publikováno v:
Experimental Cell Research. 259:308-313
Activation of caspases is the key event of apoptosis and new methods are needed to assay this event, particularly in situ, in individual cells. To measure in situ caspases activation in the present study we employed fam-VAD-fmk and fam-VEID-fmk, the
Publikováno v:
Cytometry. 41:83-88
Background During induction of apoptosis, the pro-apoptotic member of the Bcl-2 protein family (Bax) undergoes translocation to the mitochondria. The translocation, which leads to accumulation of Bax in the mitochondrial intermembrane space, appears
Publikováno v:
Cytometry. 43:38-45
Background Removal of the nucleic acid-bound fluorochrome is desirable when stained cells have to be reanalyzed using other fluorochromes. It is also often desirable to remove DNA-bound antitumor drugs from drug-treated cells, to improve cell stainin
Publikováno v:
Experimental Cell Research. 249:1-12
The laser-scanning cytometer (LSC) is a microscope-based cytofluorometer which has attributes of both flow and image cytometry. Laser-excited fluorescence emitted from fluorochromed individual cells on a microscope slide is measured at multiple wavel
Publikováno v:
Cytometry. 35:181-195
Flow cytometry techniques that are widely used in studies of cell death, and particularly in the identification of apoptotic cells, generally rely on the measurement of a single characteristic biochemical or molecular attribute. These methods fail to
Publikováno v:
Cytometry. 33:376-382
The transcriptional activator nuclear factor kappa B (NF-κB) plays a critical role in cellular response to a variety of stimuli such as pro-inflammatory cytokines, cytotoxic agents, bacterial or viral components as well as other physiological and no