Výsledky vyhledávání - "Elwin X. Vrouwe"

A generic microfluidic biosensor of G protein-coupled receptor activation - impedance measurements of reversible morphological changes of reverse transfected HEK293 cells on microelectrodes

Autor: Elwin X. Vrouwe, Saurabh K. Srivastava, R. Ramaneti, Maarten A. Jongsma, Aldo G. M. Brinkman, Hien D. Tong, Cees J.M. van Rijn, Louis C. P. M. de Smet, Margriet Roelse

Publikováno v: RSC Advances : An international journal to further the chemical sciences, 5, 52563-52570
RSC Advances : An international journal to further the chemical sciences 5 (2015)

Impedance spectroscopy of cell lines on interdigitated electrodes (IDEs) is an established method of monitoring receptor-specific cell shape changes in response to certain analytes. Normally, assays are done in multiwells making it a bulky, static an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2796cb6d4a06e727552a6a76fe12f4c7
https://research.wur.nl/en/publications/a-generic-microfluidic-biosensor-of-g-protein-coupled-receptor-ac

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A generic microfluidic biosensor of G protein-coupled receptor activation—monitoring cytoplasmic [Ca2+] changes in human HEK293 cells

Autor: Maarten A. Jongsma, Elwin X. Vrouwe, Margriet Roelse, Norbert C.A. de Ruijter

Publikováno v: Biosensors and Bioelectronics, 47, 436-444
Biosensors and Bioelectronics 47 (2013)

Cell lines expressing recombinant G-protein coupled receptors (GPCRs) are activated by specific ligands resulting in transient [Ca2+] rises that return to basal levels in 30–60 s. Yellow Cameleon 3.6 (YC3.6) is a genetically encoded calcium indicat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13e9f208aa146143490315de07d46bfe
https://research.wur.nl/en/publications/a-generic-microfluidic-biosensor-of-g-protein-coupled-receptor-ac-2

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