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pro vyhledávání: '"Elumalai, Sivamani"'
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Akademický článek
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Autor:
Qiudeng Que, Samson Nalapalli, Daolong Wang, Tara Liebler, Tim Strebe, Lee Richbourg, Elumalai Sivamani, David Bradley, Anna Prairie
Publikováno v:
Molecular biology reports. 46(3)
Phosphinothricin acetyltransferase gene (pat) is an important selectable marker and also a key herbicide trait gene in several commercial products. In maize, the transformation frequency (TF) using pat as a selectable marker is the lowest among the c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30f390d5a24bf1d20e1c0af1d35c6662
https://pubmed.ncbi.nlm.nih.gov/30859449
https://pubmed.ncbi.nlm.nih.gov/30859449
Autor:
Elumalai Sivamani, Anna Prairie, Michele Doyle, Xianggan Li, Yoshimi Barron, Qiudeng Que, David Bradley, Samson Nalapalli
Publikováno v:
Transgenic Research. 24:1017-1027
Transgenic plants containing low copy transgene insertion free of vector backbone are highly desired for many biotechnological applications. We have investigated two different strategies for increasing the percentage of low copy events in Agrobacteri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ec4e416bf9fd84ec80c64c8920f15e4
https://doi.org/10.1007/s11248-015-9902-8
https://doi.org/10.1007/s11248-015-9902-8
Sequence analysis of rice rubi3 promoter gene expression cassettes for improved transgene expression
Publikováno v:
Plant Science. 177:549-556
In a construct containing a GUS reporter gene driven by the 5′ regulatory elements from rubi3, expression was enhanced 4-fold when a 20-nucleotide (nt) GUS 5′ untranslated sequence was replaced with 9 nt sequences derived from rubi3′s second ex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5f888acea4905584452a5eaa52c57bea
https://doi.org/10.1016/j.plantsci.2009.08.006
https://doi.org/10.1016/j.plantsci.2009.08.006
Publikováno v:
Plant Cell Reports. 28:213-221
We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58c20f077b3819a282259ca51e84ee36
https://doi.org/10.1007/s00299-008-0636-4
https://doi.org/10.1007/s00299-008-0636-4
Publikováno v:
Molecular Genetics and Genomics. 279:563-572
Introns are important sequence elements that modulate the expression of genes. Using the GUS reporter gene driven by the promoter of the rice (Oryza sativa L.) polyubiquitin rubi3 gene, we investigated the effects of the 5' UTR intron of the rubi3 ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a33486e4c7646437262debbc3aefbe6e
https://doi.org/10.1007/s00438-008-0333-6
https://doi.org/10.1007/s00438-008-0333-6
Publikováno v:
Molecular Genetics and Genomics. 279:429-439
Introns play a very important role in regulating gene expression in eukaryotes. In plants, many introns enhance gene expression, and the effect of intron-mediated enhancement (IME) of gene expression is reportedly often more profound in monocots than
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c887cbd57dad9b35777b402c863f7353
https://doi.org/10.1007/s00438-008-0323-8
https://doi.org/10.1007/s00438-008-0323-8
Publikováno v:
Plant Science. 173:501-509
Tall fescue (Festuca arundinacea Schreb.) is an open-pollinated, perennial, cool-season turf and forage grass species of great economic importance. The main problems of maintenance of tall fescue, and many other turfgrasses, are two severe fungal dis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::361c2e1421042cd66956eaea4bcd08ad
https://doi.org/10.1016/j.plantsci.2007.08.002
https://doi.org/10.1016/j.plantsci.2007.08.002
Autor:
Rongda Qu, Elumalai Sivamani
Publikováno v:
Plant Molecular Biology. 60:225-239
An 808 bp promoter from a rice polyubiquitin gene, rubi3, has been isolated. The rubi3 gene contained an open reading frame of 1,140 bp encoding a pentameric polyubiquitin arranged as five tandem, head-to-tail repeats of 76 aa. The 1,140 bp 5' UTR in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b61db2628ac079899701cb262ec1ee1a
https://doi.org/10.1007/s11103-005-3853-z
https://doi.org/10.1007/s11103-005-3853-z