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pro vyhledávání: '"Elsje Sigarlakie"'
Autor:
Heinz Bauer, Elsje Sigarlakie
Publikováno v:
Journal of Ultrastructure Research. 50:208-215
By growing Saccharomyces cervisiae cells on a low phosphate medium the activity of alkaline phosphatase can be increased to a level which allows a distinct cytochemical localization of the enzyme by means of ultrathin frozen sections. Alkaline phosph
Publikováno v:
Journal of Ultrastructure Research. 51:32-39
In unstained ultrathin frozen sections of Saccharomyces cerevisiae all lipid globules show an extremely high contrast due to a strong affinity towards OsO 4 . Uranyl-acetate/lead citrate poststaining of the sections considerably decreases the contras
Autor:
Elsje Sigarlakie, Heinz Bauer
Publikováno v:
Journal of Microscopy. 99:205-218
SUMMARY Ultrathin frozen sections of glutaraldehyde fixed yeast cells have been successfully used for the demonstration of acid and alkaline phosphatase. Acid phosphatase was localized over the cell wall of both the mother cell and the bud as well as
Publikováno v:
Journal of Bacteriology. 118:747-749
A highly osmiophilic structure observed in all pigment-producing mutants of Flavobacterium examined is described. Several factors favor the possibility that this structure might be related to pigment synthesis or pigment accumulation.
Autor:
Heinz Bauer, Elsje Sigarlakie
Publikováno v:
Journal of ultrastructure research. 40(1)
A method is described to prepare ultrathin frozen sections of glutaraldehydeprefixed yeast cells. Good ultrastructural preservation of the plasmalemma, mitochondria, nuclear membrane with pores, spindle microtubules, and Golgi complex are demonstrate