Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Else G. Ankel"'
Publikováno v:
Experimental Cell Research. 150:77-83
Purified plasma fibronectin promotes the spreading of Chinese hamster ovary (CHO) cells on microcarriers in a serum-free medium. The promotion of 50% of cell spreading on microcarriers requires about 3.4 X 10(8) fibronectin molecules per bead. CHO ce
Publikováno v:
Biochemistry. 23:6393-6397
Human plasma fibronectin has been investigated by electron spin resonance (ESR) spin-label methods in conjunction with circular dichroism (CD) and sedimentation techniques to investigate its structure and flexibility in solution. The buried sulfhydry
Publikováno v:
In Vitro. 20:376-384
High concentrations of Escherichia coli asparaginase (80 U/ml) altered the binding of concanavalin A (Con A) to L 5178Y murine lymphoma cells that are sensitive to the cytotoxic action of this enzyme. Incubation of the asparaginase sensitive line in
Publikováno v:
Life sciences. 40(5)
Since nitroxide radical spin probes are used frequently to test biophysical properties of cells, their use should be restricted to conditions that do not perturb normal cell growth and viability. Eight commonly used nitroxide radical spin probes have
Publikováno v:
FEBS letters. 173(2)
The local environment of the free sulfhydryl groups in plasma fibronectin has been investigated by ESR techniques using a series of maleimide spin labels, varying in chain length between the maleimide and nitroxide free radical groups. Chemical modif
Publikováno v:
Photochemistry and photobiology. 44(6)
Spin label oximetry has been used to study the effects of photosensitizer hematoporphyrin on oxygen consumption in Chinese hamster ovary cells. These measurements demonstrate that, in the presence of the sensitizer, there occurs (i) an increase in to
Autor:
Else G. Ankel, David B. Evans, David L. Amrani, Michael W. Mosesson, Cindy M. Kane, Gene A. Homandberg
Publikováno v:
Archives of biochemistry and biophysics. 238(2)
Most (90 to 95%) human plasma fibronectin (PFn) molecules exist as 450-kDa disulfide-rich dimers comprised of two major types of subunits (A, 220 kDa; B, 215 kDa) that are joined near the COOH terminus by two disulfide bonds. Smaller PFn species (Zon
Publikováno v:
Archives of biochemistry and biophysics. 244(1)
We have examined the interaction between heparin and human plasma fibronectin using electron spin resonance (ESR) spin label methods. The titratable sulfhydryl groups of plasma fibronectin were modified with a maleimide spin label [ Lai and Tooney (1