High-Resolution IMS–MS to Assign Additional Disulfide Bridge Pairing in Complementarity-Determining Regions of an IgG4 Monoclonal Antibody

Autor: Elsa Wagner-Rousset, Olivier Colas, Kevin Giles, Sarah Cianférani, Thomas Botzanowski, Evolène Deslignière, Oscar Hernandez-Alba, Alain Beck, Dale Cooper-Shepherd, Hélène Diemer, Guillaume Béchade

Publikováno v: Journal of The American Society for Mass Spectrometry
Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), In press, ⟨10.1021/jasms.1c00151⟩
Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), In press

International audience; Monoclonal antibodies (mAbs) have taken on an increasing importance for the treatment of various diseases, including cancers and immunological disorders. Disulfide bonds play a pivotal role in therapeutic antibody structure an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d8d2127da7e4623cbd97d46b87317e4
https://doi.org/10.1021/jasms.1c00151

Fast Afucosylation Profiling of Glycoengineered Antibody Subunits by Middle-Up Mass Spectrometry

Autor: Elsa Wagner-Rousset, Yannis-Nicolas François, Stéphane Chenu, Olivier Colas, Jonathan Sjögren, Arnaud Delobel, Sarah Cianférani, Yury O. Tsybin, Davy Guillarme, Alain Beck

Publikováno v: Methods in Molecular Biology ISBN: 9781071612408
Mass Spectrometry of Glycoproteins. Methods in Molecular Biology

Middle-up LC-MS antibody characterization workflows using reduction or IdeS digestion for a focused assessment of N-glycan profiling of three representative glycoengineered monoclonal antibodies (mAbs), namely, obinutuzumab (GlycomAb technology, Glyc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8032b32dd849a463821572c7c1aa362
https://doi.org/10.1007/978-1-0716-1241-5_5

Middle-level IM-MS and CIU experiments for improved therapeutic immunoglobulin isotype fingerprinting

Autor: Olivier Colas, Thomas Botzanowski, Jean-François Haeuw, Evolène Deslignière, Oscar Hernandez-Alba, Alain Beck, Sarah Cianférani, Martine Malissard, Elsa Wagner-Rousset

Currently approved therapeutic monoclonal antibodies (mAbs) are based on immunoglobulin G (IgG) 1, 2 or 4 isotypes, which differ in their specific inter-chains disulfide bridge connectivities. Different analytical techniques have been reported for mA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e97778ef511337b40d1d4af09d8e248

Protocols for the analytical characterization of therapeutic monoclonal antibodies. I – Non-denaturing chromatographic techniques

Autor: Davy Guillarme, Valentina D'Atri, Balázs Bobály, Elsa Wagner-Rousset, Alain Beck, Szabolcs Fekete, Alexandre Goyon

Publikováno v: Journal of Chromatography. B, Vol. 1058 (2017) pp. 73-84

Size-, charge- and hydrophobicity-related variants of a biopharmaceutical product have to be deeply characterized for batch consistency and for the assessment of immunogenicity and safety effects. Size exclusion chromatography (SEC) and ion exchange

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41776d74fed93ae908e10146c5f00bff
https://doi.org/10.1016/j.jchromb.2017.05.010

Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates

Autor: Stéphane Erb, Oscar Hernandez-Alba, Elsa Wagner-Rousset, David Rabuka, Anthony Ehkirch, Alain Beck, Sarah Cianférani, Thomas Botzanowski, Olivier Colas, Penelope M. Drake

Publikováno v: mAbs
mAbs, Taylor & Francis, 2017, 9 (5), pp.801-811. ⟨10.1080/19420862.2017.1316914⟩

International audience; Antibody-drug conjugates (ADCs) have emerged as a family of compounds with promise as efficient immunotherapies. First-generation ADCs were generated mostly via reactions on either lysine side-chain amines or cysteine thiol gr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7577008c51b5473e9065e8ea4cae005a
https://doi.org/10.1080/19420862.2017.1316914