Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Elsa M. Dias"'
Autor:
Paula M. Alves, José L. Moreira, Manfred Nimtz, Manuel J.T. Carrondo, Cristina Peixoto, Helder J. Cruz, Elsa M. Dias
Publikováno v:
Biotechnology and Bioengineering. 69:129-139
BHK-21 cells expressing a human IgG-IL2 fusion protein, with potential application in tumor-targeted therapy, were grown under different nutrient conditions in a continuous system for a time period of 80 days. At very low-glucose (< 0.5 mM) or glutam
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::575cc1f80a554e793986f6d5a12c12a0
https://doi.org/10.1002/(sici)1097-0290(20000720)69:2<129::aid-bit2>3.0.co
https://doi.org/10.1002/(sici)1097-0290(20000720)69:2<129::aid-bit2>3.0.co
Autor:
Glyn N. Stacey, A. V. Savage, M. Cuffe, C. Mielke, E. Rieke, J. B. Griffiths, T. Welge, C. Burger, D. Looby, José L. Moreira, Hansjörg Hauser, Manuel J.T. Carrondo, Elsa M. Dias, K. Hayes, Helder J. Cruz
Publikováno v:
Applied Microbiology and Biotechnology. 52:345-353
Recombinant fusion proteins offer important new therapeutic approaches for the future. This report describes the use of three different genetic strategies (i.e. “mono-”, “bi-” and “tri-cistronic” vectors) to achieve stable secretion from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::426b08f2f29b6c2547400979caf05f3e
https://doi.org/10.1007/s002530051530
https://doi.org/10.1007/s002530051530
Autor:
Helder J. Cruz, José L. Moreira, Glyn Stacey, Elsa M. Dias, Katherine Hayes, Dennis Looby, Bryan Griffiths, Manuel J. T. Carrondo
Publikováno v:
Cytotechnology. 26:59-64
In this work a recombinant BHK21 clone producing a fusion protein with potential application in tumour target therapy was adapted to five different serum-free media (SFM) and to a protein-free medium (PFM). Only the PFM did not require a gradual adap
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d825a1e9c7f56bad6bb9ff90d14e8b7e
https://doi.org/10.1023/a:1007951813755
https://doi.org/10.1023/a:1007951813755
Autor:
Elsa M. Dias, J. L. Moreira, M. Nimtz, Cristina Peixoto, Helder J. Cruz, Helena Santos, H.S. Conradt, P. M. Alves, M. J. T. Carrondo
Publikováno v:
Animal Cell Technology: Products from Cells, Cells as Products ISBN: 9780792360759
BHK-21 cells expressing a rhIgG-IL2 fusion protein were grown under different nutrient conditions in a continuoussystem. At very low glucose (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9a153f3c09673ee01712844dec640acf
https://doi.org/10.1007/0-306-46875-1_55
https://doi.org/10.1007/0-306-46875-1_55
Autor:
J. L. Moreira, A. S. Ferreira, M. J. T. Carrondo, Cristina Peixoto, Elsa M. Dias, Helder J. Cruz
Publikováno v:
New Developments and New Applications in Animal Cell Technology ISBN: 0792350162
A rBHK21 clone producing an antibody/cytokine fusion protein was used to study the dependence of cell metabolism on the glucose and glutamine levels in the culture medium. Results indicate that glucose and glutamine consumptions and lactate and ammon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::caff994ae13a46579dde6a720b5ae131
https://doi.org/10.1007/0-306-46860-3_39
https://doi.org/10.1007/0-306-46860-3_39
Autor:
R. Dunker, M. Thomaz, C. Burger, A. E. Cunha, José L. Moreira, Manuel J.T. Carrondo, João J. Clemente, E. Rieke, Cristina Peixoto, Helder J. Cruz, H.S. Conradt, Elsa M. Dias
Publikováno v:
Journal of biotechnology. 96(2)
The production, purification and stability of quality (in terms of integrity and glycosylation) of an antibody/interleukin-2 fusion protein with potential application in tumour-targeted therapy expressed in BHK21 cells are described. Consistency of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54fe829702f0189a3a7605d7008b750a
https://pubmed.ncbi.nlm.nih.gov/12039533
https://pubmed.ncbi.nlm.nih.gov/12039533
Autor:
H. House, M. Beardmore, Helder J. Cruz, A. V. Savage, M. Cuffe, M. J. T. Carrondo, Elsa M. Dias, J. B. Griffiths, D. Looby, K. Hayes, Glyn N. Stacey, J. L. Moreira
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
The choice of scale up strategies for production of recombinant proteins from animal cells is often restricted to suspension cultures in stirred tank bioreactors. The primary aim of the work reported here was to identify alternative strategies for sc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0b5ce719bb8de6e4ef384b100b0fe0c8
https://doi.org/10.1007/978-94-011-5404-8_127
https://doi.org/10.1007/978-94-011-5404-8_127
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
In the present work, BHK cells producing a recombinant protein with potential application in tumor target therapy were used in order to study the effect of operating conditions upon growth, production and product quality. Cells were grown in a protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8ed76959fb20714b0d6fa6f8f11ff7d8
https://doi.org/10.1007/978-94-011-5404-8_113
https://doi.org/10.1007/978-94-011-5404-8_113
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
In this work, a BHK21 clone producing a fusion protein with potential application in tumor target therapy was adapted to several serum-free media. Three different serum-free media (SFM) were tested (QBSF 56, CHO-S-SFMH, DMEM/Ham’s F12 derived mediu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::66a5e546a70658032af7cb19b6cbb67f
https://doi.org/10.1007/978-94-011-5404-8_112
https://doi.org/10.1007/978-94-011-5404-8_112