Design of protein-binding peptides with controlled binding affinity: the case of SARS-CoV-2 receptor binding domain and angiotensin-converting enzyme 2 derived peptides

Autor: Giacomo Parisi, Roberta Piacentini, Alessio Incocciati, Alessandra Bonamore, Alberto Macone, Jakob Rupert, Elsa Zacco, Mattia Miotto, Edoardo Milanetti, Gian Gaetano Tartaglia, Giancarlo Ruocco, Alberto Boffi, Lorenzo Di Rienzo

Publikováno v: Frontiers in Molecular Biosciences, Vol 10 (2024)

The development of methods able to modulate the binding affinity between proteins and peptides is of paramount biotechnological interest in view of a vast range of applications that imply designed polypeptides capable to impair or favour Protein-Prot

Externí odkaz: https://doaj.org/article/7a5d138e1b4c4cc189f2c031fc731c96

Probing TDP-43 condensation using an in silico designed aptamer

Autor: Elsa Zacco, Owen Kantelberg, Edoardo Milanetti, Alexandros Armaos, Francesco Paolo Panei, Jenna Gregory, Kiani Jeacock, David J. Clarke, Siddharthan Chandran, Giancarlo Ruocco, Stefano Gustincich, Mathew H. Horrocks, Annalisa Pastore, Gian Gaetano Tartaglia

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)

Here, the authors generated an artificial RNA molecule, or aptamer, specific for the Amyotrophic Lateral Sclerosis protein TDP-43. By interacting avidly with its target, the aptamer can be exploited to track TDP-43 phase transition in vitro and in ce

Externí odkaz: https://doaj.org/article/313504fc94a146ba963e22a3b9d3a822

The Role of Glycation on the Aggregation Properties of IAPP

Autor: Giulia Milordini, Elsa Zacco, Matthew Percival, Rita Puglisi, Fabrizio Dal Piaz, Pierandrea Temussi, Annalisa Pastore

Publikováno v: Frontiers in Molecular Biosciences, Vol 7 (2020)

Epidemiological evidence shows an increased risk for developing Alzheimer’s disease in people affected by diabetes, a pathology associated with increased hyperglycemia. A potential factor that could explain this link could be the role that sugars m

Externí odkaz: https://doaj.org/article/dbb8bf2807dc4df296c157a1221b80b8

Glycans to improve efficacy and solubility of protein aggregation inhibitors

Autor: Ashim Paul, Daniel Segal, Elsa Zacco

Publikováno v: Neural Regeneration Research, Vol 16, Iss 11, Pp 2215-2216 (2021)

Externí odkaz: https://doaj.org/article/c8acd4efbee849ebbd4b5247c493092d

Single‐Molecule Two‐Color Coincidence Detection of Unlabeled alpha‐Synuclein Aggregates

Autor: Alexandre Chappard, Craig Leighton, Rebecca S. Saleeb, Kiani Jeacock, Sarah R. Ball, Katie Morris, Owen Kantelberg, Ji‐Eun Lee, Elsa Zacco, Annalisa Pastore, Margaret Sunde, David J. Clarke, Patrick Downey, Tilo Kunath, Mathew H. Horrocks

Publikováno v: Chappard, A, Leighton, C, Saleeb, R S, Jeacock, K, Ball, S, Morris, K, Kantelberg, O, Lee, J E, Zacco, E, Pastore, A, Sunde, M, Clarke, D J, Downey, P, Kunath, T & Horrocks, M H 2023, ' Single-Molecule Two-Color Coincidence Detection of Unlabeled alpha-Synuclein Aggregates ', Angewandte Chemie-International Edition, vol. 62, no. 15, e202216771 . https://doi.org/10.1002/anie.202216771

Protein misfolding and aggregation into oligomeric and fibrillar structures is a common feature of many neurogenerative disorders. Single-molecule techniques have enabled characterization of these lowly abundant, highly heterogeneous protein aggregat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fe643023fcf8b99b1fc91725dd0e2a9
https://doi.org/10.1002/anie.202216771