Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Elodie Bertosio"'
Autor:
Cédric Sapet, Nicolas Laurent, Antoine de Chevigny, Loïc Le Gourrierec, Elodie Bertosio, Olivier Zelphati, Christophe Béclin
Publikováno v:
BioTechniques, Vol 62, Iss 1, p xii (2017)
Protocol Summary Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well a
Externí odkaz:
https://doaj.org/article/34626dc70f404f9cbe3963400e8ae65a
Autor:
Cédric Sapet, Nicolas Laurent, Antoine de Chevigny, Loïc Le Gourrierec, Elodie Bertosio, Olivier Zelphati, Christophe Béclin
Publikováno v:
BioTechniques, Vol 50, Iss 3, Pp 187-189 (2011)
Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throug
Externí odkaz:
https://doaj.org/article/87d769adc6f2417d8dad34b74cc827a4
Autor:
Tadashi Yokosuka, Romain Roncagalli, Yinming Liang, Bernard Malissen, Aurélie Malzac, Jean Imbert, Christoph Wülfing, Isaac J Nijman, Margot Cucchetti, Takashi Saito, Marie Malissen, Miloslav Suchanek, Elodie Bertosio
Publikováno v:
Nature Immunology
Although T cell activation can result from signaling via T cell antigen receptor (TCR) alone, physiological T cell responses require costimulation via the coreceptor CD28. Through the use of an N-ethyl-N-nitrosourea-mutagenesis screen, we identified
Autor:
Nicolas Laurent, Cédric Sapet, Elodie Bertosio, Olivier Zelphati, Cécile Formosa, Flavie Sicard
Publikováno v:
Therapeutic Delivery
Background: 3D matrices are widely used as cell growth supports in basic research, regenerative medicine or cell-based drug assays. In order to genetically manipulate cells cultured within 3D matrices, two novel non-viral transfection reagents allowi
Autor:
Mélanie Bertuzzi, Flavie Sicard, Cédric Sapet, Elodie Bertosio, Olivier Zelphati, Nicolas Laurent
Publikováno v:
Minicircle and Miniplasmid DNA Vectors: The Future of Nonviral and Viral Gene Transfer
Autor:
David A. Mancardi, Diana Ordoñez-Rueda, Martin Hrabě de Angelis, Wei-Dong Zhao, Véronique Blanquet, Pierre Bruhns, Yinming Liang, Friederike Jönsson, Bernard Malissen, Elodie Bertosio, Stéphane Méresse, Aurélie Malzac, Estelle Duprez, Pierre Grenot, Marie Malissen, Sybille Sabrautzki
Publikováno v:
European Journal of Immunology. 42:2395-2408
Using N-ethyl-N-nitrosourea-induced mutagenesis, we established a mouse model with a novel form of neutropenia resulting from a point mutation in the transcriptional repressor Growth Factor Independence 1 (Gfi1). These mice, called Genista, had norma
Autor:
Enrique Aguado, Amandine Sansoni, Arkadiusz Miazek, Michael Mingueneau, Marie Malissen, Elodie Bertosio, Pierre Perrin, Bernard Malissen, Adrien Kissenpfennig, Cristel Archambaud, Sylvie Richelme, Romain Roncagalli, Claude Grégoire, Ying Wang, Richard M. Locksley
Publikováno v:
Immunity
Immunity, Elsevier, 2009, 31 (2), pp.197-208. ⟨10.1016/j.immuni.2009.05.013⟩
Immunity; Vol 31
Immunity, 2009, 31 (2), pp.197-208. ⟨10.1016/j.immuni.2009.05.013⟩
Mingueneau, M, Roncagalli, R, Grégoire, C, Kissenpfennig, A, Miazek, A, Archambaud, C, Wang, Y, Perrin, P, Bertosio, E, Sansoni, A, Richelme, S, Locksley, R M & Aguado, E 2009, ' Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor. ', Immunity, vol. 31(2), no. 2, pp. 197-208 . https://doi.org/10.1016/j.immuni.2009.05.013
Immunity, Elsevier, 2009, 31 (2), pp.197-208. ⟨10.1016/j.immuni.2009.05.013⟩
Immunity; Vol 31
Immunity, 2009, 31 (2), pp.197-208. ⟨10.1016/j.immuni.2009.05.013⟩
Mingueneau, M, Roncagalli, R, Grégoire, C, Kissenpfennig, A, Miazek, A, Archambaud, C, Wang, Y, Perrin, P, Bertosio, E, Sansoni, A, Richelme, S, Locksley, R M & Aguado, E 2009, ' Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor. ', Immunity, vol. 31(2), no. 2, pp. 197-208 . https://doi.org/10.1016/j.immuni.2009.05.013
International audience; Despite compromised T cell antigen receptor (TCR) signaling, mice in which tyrosine 136 of the adaptor linker for activation of T cells (LAT) was constitutively mutated (Lat(Y136F) mice) accumulate CD4(+) T cells that trigger
Autor:
Nicolas Laurent, Olivier Zelphati, Cédric Sapet, Loïc Le Gourrierec, Elodie Bertosio, Christophe Beclin, Antoine de Chevigny
Publikováno v:
BioTechniques. 62:xii
Protocol Summary Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well a
Publikováno v:
Therapeutic delivery. 2(4)
In recent years, gene therapy has received considerable interest as a potential method for the treatment of numerous inherited and acquired diseases. However, successes have so far been hampered by several limitations, including safety issues of vira
Publikováno v:
Protocol Exchange.