Visualizing the transiently populated closed-state of human HSP90 ATP binding domain

Autor: Faustine Henot, Elisa Rioual, Adrien Favier, Pavel Macek, Elodie Crublet, Pierre Josso, Bernhard Brutscher, Matthias Frech, Pierre Gans, Claire Loison, Jerome Boisbouvier

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)

To refold client proteins, HSP90 chaperone undergoes large structural rearrangements. Here the authors use NMR and molecular simulation and reveal structure and dynamics of a key functionally relevant metastable state of human HSP90α N-terminal doma

Externí odkaz: https://doaj.org/article/a0e415192adb4a8f825b0f4fa98dd24f

Immunogenicity of the Plasmodium falciparum PfEMP1-VarO Adhesin: Induction of Surface-Reactive and Rosette-Disrupting Antibodies to VarO Infected Erythrocytes.

Autor: Micheline Guillotte, Alexandre Juillerat, Sébastien Igonet, Audrey Hessel, Stéphane Petres, Elodie Crublet, Cécile Le Scanf, Anita Lewit-Bentley, Graham A Bentley, Inès Vigan-Womas, Odile Mercereau-Puijalon

Publikováno v: PLoS ONE, Vol 10, Iss 7, p e0134292 (2015)

Adhesion of Plasmodium falciparum-infected red blood cells (iRBC) to human erythrocytes (i.e. rosetting) is associated with severe malaria. Rosetting results from interactions between a subset of variant PfEMP1 (Plasmodium falciparum erythrocyte memb

Externí odkaz: https://doaj.org/article/cff3771abc2046d38bc0f9436991bc3e

In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies

Autor: Rime Kerfah, Emeric Miclet, Pierre Gans, Céline Juillan-Binard, Elodie Crublet, Alicia Vallet, Rachel Lenoir-Capello, Lionel Imbert, Jérôme Boisbouvier, Hubert Mayerhofer, Rida Awad, Isabel Ayala

Publikováno v: Methods in Molecular Biology ISBN: 9781071608913
Methods in Molecular Biology
Methods in Molecular Biology, 2021, 2199, pp.127-149. ⟨10.1007/978-1-0716-0892-0_8⟩
Methods in Molecular Biology-Structural Genomics
Structural Genomics-General Applications
Methods in Molecular Biology, Humana Press/Springer Imprint, 2021, 2199, pp.127-149. ⟨10.1007/978-1-0716-0892-0_8⟩

International audience; The cell-free synthesis is an efficient strategy to produce in large scale protein samples for structural investigations. In vitro synthesis allows for significant reduction of production time, simplification of purification s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71677b64694f2c37e6793ea04a003290
https://doi.org/10.1007/978-1-0716-0892-0_8

Computational reverse-engineering of a spider-venom derived peptide active against Plasmodium falciparum SUB1.

Autor: Giacomo Bastianelli, Anthony Bouillon, Christophe Nguyen, Elodie Crublet, Stéphane Pêtres, Olivier Gorgette, Dung Le-Nguyen, Jean-Christophe Barale, Michael Nilges

Publikováno v: PLoS ONE, Vol 6, Iss 7, p e21812 (2011)

BACKGROUND: Psalmopeotoxin I (PcFK1), a protein of 33 aminoacids derived from the venom of the spider Psalmopoeus Cambridgei, is able to inhibit the growth of Plasmodium falciparum malaria parasites with an IC50 in the low micromolar range. PcFK1 was

Externí odkaz: https://doaj.org/article/9aea6e5faaf34cb19d66cc6fa0250c4a

Unraveling self-assembly pathways of the 468-kDa proteolytic machine TET2

Autor: Pavel Macek, Guy Schoehn, Rime Kerfah, Elodie Crublet, Jérôme Boisbouvier, Elisabetta Boeri Erba, Christine Moriscot, Carlos Amero

Publikováno v: Science Advances
Science Advances, American Association for the Advancement of Science (AAAS), 2017, 3 (4), pp.e1601601. ⟨10.1126/sciadv.1601601⟩
Science Advances, 2017, 3 (4), pp.e1601601. ⟨10.1126/sciadv.1601601⟩

Real-time NMR, EM, and native MS studies revealed intermediates and parallel pathways in the assembly of a dodecameric peptidase.
The spontaneous formation of biological higher-order structures from smaller building blocks, called self-assembly,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::320fa2ac44fb8c122ffce58f7a11db35
http://europepmc.org/articles/PMC5384809