Výsledky vyhledávání - "Ellert R. Nichols"

Effect of Size, Quaternary Structure and Translational Error on the Static and Dynamic Heterogeneity of β-Galactosidase and Measurement of Electrophoretic Dynamic Heterogeneity

Autor: Ellert R. Nichols, Allison M. HaslamA.M. Haslam, Elnaz Shadabi, Miki Chikamatsu, Douglas B. Craig, H. J. Silverstein

Publikováno v: The Protein Journal. 29:398-406

Single enzyme molecule assays were performed using capillary electrophoresis-based protocols on beta-galactosidase from Lactobacillus delbrueckii, Lactobacillus reuteri, Lactobacillus helveticus and Bacillus circulans. The enzyme was found to show st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56dfbe513bbab4e9442c46f5eb11fe9e
https://doi.org/10.1007/s10930-010-9266-0

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Continuous flow assay for the simultaneous measurement of the electrophoretic mobility, catalytic activity and its variation over time of individual molecules ofEscherichia coliβ-galactosidase

Autor: Ellert R. Nichols, Douglas B. Craig

Publikováno v: ELECTROPHORESIS. 29:4298-4303

A CE-LIF detection-based assay was developed for the study of individual molecules of Escherichia coli beta-galactosidase. The assay allows for the simultaneous measurement of the electrophoretic mobility, catalytic activity and the variation in acti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ede0dd3dbd175f28259161fadf67122
https://doi.org/10.1002/elps.200800482

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Effect of alteration of translation error rate on enzyme microheterogeneity as assessed by variation in single molecule electrophoretic mobility and catalytic activity

Autor: Elnaz Shadabi, Douglas B. Craig, Ellert R. Nichols

Publikováno v: Biochemistry and cell biology = Biochimie et biologie cellulaire. 87(3)

The role of translation error for Escherichia coli individual β-galactosidase molecule catalytic and electrophoretic heterogeneity was investigated using CE-LIF. An E. coli rpsL mutant with a hyperaccurate translation phenotype produced enzyme molec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12e5a3c13f5faaff27cd284c6451ee44
https://pubmed.ncbi.nlm.nih.gov/19448745

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Single molecule assays reveal differences between in vitro and in vivo synthesized beta-galactosidase

Autor: Douglas B. Craig, Ellert R. Nichols

Publikováno v: The protein journal. 27(6)

Genomic DNA was extracted from wild-type Escherichia coli strains ATCC 35321 and 8677. The lac Z gene was amplified and used as a template for in vitro synthesis of beta-galactosidase. In addition the enzyme was synthesized in vitro with a C-terminal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d7a525217d57f846a12bdce47b27cdb
https://pubmed.ncbi.nlm.nih.gov/18769886

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Single molecule assays of beta-galactosidase from two wild-type strains of E. coli: effects of protease inhibitors on microheterogeneity and different relative activities with differing substrates

Autor: Ellert R. Nichols, Douglas B. Craig, Jennilee M. A. Gavina, Robert G. McLeod

Publikováno v: The protein journal. 26(2)

Beta-galactosidase was induced in E. coli wild type strains ATCC 8677 and 35321 in the presence of various protease inhibitors. Single enzyme molecule assays were performed using a capillary electrophoresis based protocol. The presence of the proteas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75ab79a531fff5edbb77e387bc964311
https://pubmed.ncbi.nlm.nih.gov/17253128

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Spectroscopic Measurement of the Redox Potential of Cytochrome c for the Undergraduate Biochemistry Laboratory

Autor: Ellert R. Nichols, Douglas B. Craig

Publikováno v: Journal of Chemical Education. 83:1325

Teaching redox chemistry is an important facet of undergraduate biochemistry courses, particularly with respect to developing the students' understanding of the central metabolic processes such as the mitochondrial respiratory chain and photosynthesi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3700316e90b4ac9816c8e5a81df0f223
https://doi.org/10.1021/ed083p1325

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