Going Back and Forth: Episomal Vector Reprogramming of Peripheral Blood Mononuclear Cells to Induced Pluripotent Stem Cells and Subsequent Differentiation into Cardiomyocytes and Neuron-Astrocyte Co-cultures

Autor: Victoria C. de Leeuw, Conny T. M. van Oostrom, Martijn E.T. Dollé, Sandra Imholz, Aldert H. Piersma, Ellen V.S. Hessel

Publikováno v: Cellular Reprogramming

Human induced pluripotent stem cells (iPSCs) can capture the diversity in the general human population as well as provide deeper insight in cellular mechanisms. This makes them suitable to study both fundamental and applied research subjects, such as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ef01fc72358611b5a8de17b716b752c
http://europepmc.org/articles/PMC7757589

Neuronal Differentiation Pathways and Compound-Induced Developmental Neurotoxicity in the Human Neural Progenitor Cell Test (Hnpt) Revealed by Rna-Seq

Autor: Victoria C. de Leeuw, Conny T.M. van Oostrom, Paul F.K. Wackers, Jeroen L.A. Pennings, Hennie M. Hodemaekers, Aldert H. Piersma, Ellen V.S. Hessel

Publikováno v: Chemosphere

There is an increased awareness that the use of animals for compound-induced developmental neurotoxicity (DNT) testing has limitations. Animal-free innovations, especially the ones based on human stem cell-based models are pivotal in studying DNT sin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cf8f254f07ba555a318f5bf3ac5fa3b
https://doi.org/10.2139/ssrn.4048728

Developmental Neurotoxicity of Environmentally Relevant Pharmaceuticals and Mixtures Thereof in a Zebrafish Embryo Behavioural Test

Autor: Edwin P. Zwart, Ingrid Jense, Harm J. Heusinkveld, Jessica Legradi, Ellen V.S. Hessel, Alessandro Atzei, Bastiaan J. Venhuis, Leo T.M. van der Ven

Publikováno v: International Journal of Environmental Research and Public Health
Volume 18
Issue 13
International Journal of Environmental Research and Public Health, Vol 18, Iss 6717, p 6717 (2021)
Atzei, A, Jense, I, Zwart, E P, Legradi, J, Venhuis, B J, van der Ven, L T M, Heusinkveld, H J & Hessel, E V S 2021, ' Developmental neurotoxicity of environmentally relevant pharmaceuticals and mixtures thereof in a zebrafish embryo behavioural test ', International Journal of Environmental Research and Public Health, vol. 18, no. 13, 6717 . https://doi.org/10.3390/ijerph18136717
International Journal of Environmental Research and Public Health, 18(13):6717. Multidisciplinary Digital Publishing Institute (MDPI)

Humans are exposed daily to complex mixtures of chemical substances via food intake, inhalation, and dermal contact. Developmental neurotoxicity is an understudied area and entails one of the most complex areas in toxicology. Animal studies for devel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77fe1c045c17ab6a0ea90c7046286d3b

Exploring the biological domain of the neural embryonic stem cell test (ESTn): Morphogenetic regulators, Hox genes and cell types, and their usefulness as biomarkers for embryotoxicity screening

Autor: Aldert H. Piersma, Jeroen L. A. Pennings, Victoria C. de Leeuw, Ellen V.S. Hessel

Publikováno v: Toxicology. 454

Animal-free assessment of compound-induced developmental neurotoxicity will most likely be based on batteries of multiple in vitro tests. The optimal battery is built by combining tests with complementary biological domains that together ideally cove

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b4337b316f92910dc960925e03934d8
https://pubmed.ncbi.nlm.nih.gov/33636252

Differential effects of fluoxetine and venlafaxine in the neural embryonic stem cell test (ESTn) revealed by a cell lineage map

Autor: Conny T. M. van Oostrom, Paul F.K. Wackers, Ellen V.S. Hessel, Hennie M. Hodemaekers, Jeroen L. A. Pennings, Aldert H. Piersma, Victoria C. de Leeuw

Publikováno v: Neurotoxicology. 76

There is a need for in vitro tests for the evaluation of chemicals and pharmaceuticals that may cause developmental neurotoxicity (DNT) in humans. The neural embryonic stem cell test (ESTn) is such an in vitro test that mimics early neural differenti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bc04bd4aa2d2c0c4f7a2a71a345b2bc
https://pubmed.ncbi.nlm.nih.gov/31593710