Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Ellen Nordman"'
Autor:
Fuchou Tang, Catalin Barbacioru, Ellen Nordman, Siqin Bao, Caroline Lee, Xiaohui Wang, Brian B Tuch, Edith Heard, Kaiqin Lao, M Azim Surani
Publikováno v:
PLoS ONE, Vol 6, Iss 6, p e21208 (2011)
Stochastic and deterministic allele specific gene expression (ASE) might influence single cell phenotype, but the extent and nature of the phenomenon at the onset of early mouse development is unknown. Here we performed single cell RNA-Seq analysis o
Externí odkaz:
https://doaj.org/article/fd2ac4e7b75244b8bc77b5063a64f55a
Autor:
Ellen Nordman, Kaiqin Lao, M. Azim Surani, Vladimir I. Bashkirov, Catalin Barbacioru, Fuchou Tang, Bin Li, Nanlan Xu
Publikováno v:
Nature Protocols. 5:516-535
We describe here a protocol for digital transcriptome analysis in a single mouse blastomere using a deep sequencing approach. An individual blastomere was first isolated and put into lysate buffer by mouth pipette. Reverse transcription was then perf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f1f47673f7970150accae2f301e6fbd
https://doi.org/10.1038/nprot.2009.236
https://doi.org/10.1038/nprot.2009.236
Autor:
Clarence Lee, Xiaohui Wang, Asim Siddiqui, Brian B. Tuch, John Bodeau, Fuchou Tang, Catalin Barbacioru, Yangzhou Wang, Nanlan Xu, M. Azim Surani, Ellen Nordman, Kaiqin Lao
Publikováno v:
Nature Methods. 6:377-382
Next-generation sequencing technology is a powerful tool for transcriptome analysis. However, under certain conditions, only a small amount of material is available, which requires more sensitive techniques that can preferably be used at the single-c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f69748f0be9904a222b2b457e343bb37
https://doi.org/10.1038/nmeth.1315
https://doi.org/10.1038/nmeth.1315
Autor:
Catalin Barbacioru, Fuchou Tang, M. Azim Surani, Siqin Bao, Ellen Nordman, Edith Heard, Kaiqin Lao, Brian B. Tuch, Caroline Lee, Xiaohui Wang
Publikováno v:
PLoS ONE
PLoS ONE, Vol 6, Iss 6, p e21208 (2011)
PLoS ONE, Vol 6, Iss 6, p e21208 (2011)
Stochastic and deterministic allele specific gene expression (ASE) might influence single cell phenotype, but the extent and nature of the phenomenon at the onset of early mouse development is unknown. Here we performed single cell RNA-Seq analysis o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::588ffacc3fbeff199d3446bb9ce761a6
http://europepmc.org/articles/PMC3121735
http://europepmc.org/articles/PMC3121735
Autor:
Kai Q, Lao, Fuchou, Tang, Catalin, Barbacioru, Yangzhou, Wang, Ellen, Nordman, Clarence, Lee, Nanlan, Xu, Xiaohui, Wang, Brain, Tuch, John, Bodeau, Asim, Siddiqui, M Azim, Surani
Publikováno v:
Journal of biomolecular techniques : JBT. 20(5)
We have developed a sequencing-based gene expression profiling assay at single-cell resolution by combining a modified single-cell whole transcriptome amplification method with the next generation sequencing technique, the SOLiD™ system. Using this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3a4d31303be80bf3a4ce1242145c1cd0
https://pubmed.ncbi.nlm.nih.gov/19949700
https://pubmed.ncbi.nlm.nih.gov/19949700
Autor:
Kaiqin Lao, Caroline Lee, Xiaohui Wang, Ellen Nordman, M. Azim Surani, Fuchou Tang, Siqin Bao, Catalin Barbacioru
Publikováno v:
Cell Stem Cell
Summary During the transition from the inner cell mass (ICM) cells of blastocysts to pluripotent embryonic stem cells (ESCs) in vitro, a normal developmental program is replaced in cells that acquire a capacity for infinite self-renewal and pluripote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::000ace9af9a1bb5c70f02ff3c7acd45b