Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Ella Bedford"'
Autor:
Rongjie Fu, Wei He, Jinzhuang Dou, Oscar D. Villarreal, Ella Bedford, Helen Wang, Connie Hou, Liang Zhang, Yalong Wang, Dacheng Ma, Yiwen Chen, Xue Gao, Martin Depken, Han Xu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
The sequence determinants governing CRISPR/Cas9 specificity are not fully understood. Here, the authors devise a high-throughput dual-target synthetic system to explore the sequence features associated with CRISPR/Cas9 off-target effect, reveal a set
Externí odkaz:
https://doaj.org/article/5fc93d0311ad442eb76be261e9de5028
Autor:
Wei He, Liang Zhang, Oscar D. Villarreal, Rongjie Fu, Ella Bedford, Jingzhuang Dou, Anish Y. Patel, Mark T. Bedford, Xiaobing Shi, Taiping Chen, Blaine Bartholomew, Han Xu
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.
Externí odkaz:
https://doaj.org/article/8f9bec4646d34e8fa3bf3716402d6050
Autor:
Swarnalatha Manickavinayaham, Renier Vélez-Cruz, Anup K. Biswas, Ella Bedford, Brianna J. Klein, Tatiana G. Kutateladze, Bin Liu, Mark T. Bedford, David G. Johnson
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
E2F1, which localises to DNA double-strand breaks (DSBs) to promote repair, is acetylated in response to DNA damage but the role this plays in DNA repair is unknown. Here the authors show that E2F1 acetylation creates a binding motif for the bromodom
Externí odkaz:
https://doaj.org/article/c922be47af0c487c9f56067db1a6901f
Autor:
Wei He, Liang Zhang, Oscar D. Villarreal, Rongjie Fu, Ella Bedford, Jingzhuang Dou, Anish Y. Patel, Mark T. Bedford, Xiaobing Shi, Taiping Chen, Blaine Bartholomew, Han Xu
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-1 (2020)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Externí odkaz:
https://doaj.org/article/c73123d16f894556a2f4a07d0df664e6
Autor:
Liang Zhang, Taiping Chen, Han Xu, Xiaobing Shi, Mark T. Bedford, Jingzhuang Dou, Blaine Bartholomew, Wei He, Ella Bedford, Rongjie Fu, Anish Y. Patel, Oscar D. Villarreal
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
Nature Communications
Nature Communications
High-throughput CRISPR-Cas9 knockout screens using a tiling-sgRNA design permit in situ evaluation of protein domain function. Here, to facilitate de novo identification of essential protein domains from such screens, we propose ProTiler, a computati
Autor:
Rongjie Fu, Wei He, Jinzhuang Dou, Oscar D. Villarreal, Ella Bedford, Helen Wang, Connie Hou, Liang Zhang, Yalong Wang, Dacheng Ma, Yiwen Chen, Xue Gao, Martin Depken, Han Xu
Publikováno v:
Nature Communications
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Nature Communications, 13(1)
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Nature Communications, 13(1)
The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (gRNA) and the targeted DNA, yet the sequence-dependent rules underlying off-target effects are not fully understood. To systematically explore the sequ
Autor:
David G. Johnson, Anup K. Biswas, Tatiana G. Kutateladze, Swarnalatha Manickavinayaham, Renier Vélez-Cruz, Brianna J. Klein, Mark T. Bedford, Bin Liu, Ella Bedford
Publikováno v:
Nature Communications
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
E2F1 and retinoblastoma (RB) tumor-suppressor protein not only regulate the periodic expression of genes important for cell proliferation, but also localize to DNA double-strand breaks (DSBs) to promote repair. E2F1 is acetylated in response to DNA d
Autor:
Han Xu, Ella Bedford, Oscar D. Villarreal, Rongjie Fu, Liang Zhang, Xiaobing Shi, Wei He, Blaine Bartholomew, Mark T. Bedford, Jingzhuang Dou, Taiping Chen
High-throughput CRISPR/Cas9 knockout screens using a tiling-sgRNA design permit in situ evaluation of protein domain function. To facilitate de novo identification of essential protein domains from such screens, we developed ProTiler, a computational
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06da392859e57b636eee5c03cb6ed26d
https://doi.org/10.1101/581751
https://doi.org/10.1101/581751
Autor:
Xiaobing Shi, Blaine Bartholomew, Taiping Chen, Anish Y. Patel, Han Xu, Liang Zhang, Jingzhuang Dou, Rongjie Fu, Ella Bedford, Mark T. Bedford, Oscar D. Villarreal, Wei He
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-1 (2020)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Autor:
K. Leslie Powell, Howard D. Thames, Nikki Liburd, Lea Spyres, Stacey Arantes, Michael C. MacLeod, Ella Bedford, Earl F. Walborg, Mahmoud Rouabhia, C. Marcelo Aldaz, David L. Mitchell, Sally Gaddis
Publikováno v:
Analytical Biochemistry. 345:284-295
Microarray technologies have provided the ability to monitor the expression of whole genomes rapidly. However, concerns persist with regard to quantitation and reproducibility, and the detection limits for individual genes in particular arrays are ge