Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Elke Oudenampsen"'
Autor:
Nils J. Færgeman, B. Rolf, Axel Lezius, Jens Knudsen, Friedrich Spener, Elke Oudenampsen-Krüger, Torsten Börchers
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1259:245-253
The coding part of the cDNA for bovine liver-type fatty acid binding protein (L-FABP) has been amplified by RT-PCR, cloned and used for the construction of an Escherichia coli (E. coli) expression system. The recombinant protein made up to 25% of the
Autor:
Franz Hillenkamp, Friedrich Spener, Arnd Ingendoh, Elke Oudenampsen-Krüger, Bernfried Specht, Axel Lezius
Publikováno v:
Journal of biotechnology. 33(3)
An expression vector for bovine heart fatty acid-binding protein (H-FABP) was constructed by introducing the coding part of the cDNA into the pET-3d vector. Transformed Escherichia coli strain BL21 (DE3)pLysS produced functional recombinant H-FABP up
Publikováno v:
Molecular and cellular biochemistry. 98(1-2)
The coding part of the cDNA of cardiac fatty acid-binding protein (cFABP) from bovine heart was cloned into the vector pKK233-2. After induction with isopropyl-beta-D-thiogalactopyranoside cFABP was found in a soluble form in the cytosol of plasmid t