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Expression of human papilloma virus type 16 antigens, specific targeting as well as formation of virus-like particles by HSV-1 amplicon vectors

Autor: Alberto L. Epstein, Sabine Schenck, Charles W. Knopf, Elke Kehm, Martin Müller, Hanswalter Zentgraf

Publikováno v: Virus Genes. 37:131-143

Expression of human papilloma virus type 16 (HPV16) antigens by herpes simplex virus type 1 (HSV-1)-based amplicon vectors was investigated. Amplicons were packaged using HSV-1 LaL, a virus with a floxed 'a' sequence, and the CRE recombinase-expressi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50ef86f321944a9bd3de6d606b8b0d28
https://doi.org/10.1007/s11262-008-0247-8

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Herpes simplex Virus Type 1 DNase: Functional Analysis of the Enzyme Expressed by Recombinant Baculovirus

Autor: Mehmet-Ali Göksu, Stefanie Bayer, Charles W. Knopf, Elke Kehm

Publikováno v: Intervirology. 41:110-119

Herpes simplex virus type 1 DNase (HSV-1 DNase) was expressed in insect cells by recombinant baculovirus (NPVUL12) and purified by a combination of anionic exchanger chromatography and gel filtration. Two polypeptides of 85 and 75 kD, whose ratio var

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd04078425ab3611266815b2df8a39b8
https://doi.org/10.1159/000024922

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[Untitled]

Autor: Elke Kehm, Göksu Ma, Knopf Cw

Publikováno v: Virus Genes. 17:129-138

Expression of recombinant herpes simplex virus type 1 (HSV-1) deoxyribonuclease (DNase) was analyzed in BHK-21 cells, a standard cell line for virus propagation, by using mammalian cell expression systems based on vaccinia virus and on Semliki Forest

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::075c50657fa4dd4ccf64d4ac6c9253c9
https://doi.org/10.1023/a:1008012606497

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One-step column purification of herpes simplex virus 1 helicase-primase subcomplex using C-terminally his-tagged UL5 subunit

Autor: Charles W. Knopf, Myriam Theune, Elke Kehm, Uwe Schreiner, Frank Althof

Publikováno v: Virus genes. 39(1)

A protocol for purification of the two-subunit complex of herpes simplex virus type 1 (HSV-1) helicase-primase by metal affinity chromatography is presented. In order to bind the enzyme complex consisting of UL5 and UL52 gene functions to the affinit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::253319ed8f78c941df4b31042657470d
https://pubmed.ncbi.nlm.nih.gov/19396588

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