Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Elizabeth M. Furter-Graves"'
Publikováno v:
Protein Science. 5:320-330
Creatine kinase (CK) has been postulated to consist of two flexibly hinged domains. A previously demonstrated protease-sensitive site in M-CK (Morris & Jackson, 1991) has directed our attempts to dissect mitochondrial CK (Mi-CK) into two protein frag
Publikováno v:
Protein Science. 3:1058-1068
The 5 tryptophan residues of chicken sarcomeric mitochondrial creatine kinase (Mib-CK) were individually replaced by phenylalanine or cysteine using site-directed mutagenesis. The mutant proteins were analyzed by enzyme kinetics, fluorescence spectro
Autor:
Hans M. Eppenberger, Wolfram Hemmer, Elizabeth M. Furter-Graves, Else Zanolla, Theo Wallimann
Publikováno v:
European Journal of Neuroscience. 6:538-549
Creatine kinase isoenzymes were localized in the chicken cerebellum by the use of isoenzyme-specific anti-chicken creatine kinase antibodies. Brain-type creatine kinase was found in high amounts in the molecular layer, particularly in Bergmann glial
Publikováno v:
Nucleic Acids Research. 22:4932-4936
The yeast shi mutation affects the spacing between the TATA promoter element and transcription initiation sites; for the H2B and ADH1 genes, a series of start sites located approximately 50-80 bp downstream of TATA is used in addition to the wild-typ
Publikováno v:
Biochemistry. 32:7022-7029
Chemical modification of rabbit muscle creatine kinase (CK) with thiol-specific reagents led to partial or complete inactivation of the enzyme. Using site-directed mutagenesis, we have substituted the corresponding reactive Cys278 in the chicken card
Publikováno v:
Biochimica et biophysica acta. 1251(2)
We report that several different chicken and rabbit creatine kinase (CK)1 isoenzymes showed an incorporation of 32P when incubated with [γ-32P]ATP in an autophosphorylation assay. This modification was shown to be of covalent nature and resulted fro
Publikováno v:
Moleculargeneral genetics : MGG. 223(3)
The roles of the TATA element and sequences near the mRNA initiation site in specifying the location of initiation sites in Saccharomyces cerevisiae were examined, using the Schizosaccharomyces pombe ADH gene. The importance of spacing was demonstrat
Publikováno v:
Cell. 61:379-380
Autor:
Philipp Kaldis, Rolf Furter, Theo Wallimann, Elizabeth M. Furter-Graves, Hans M. Eppenberger, Thomas Schnyder
Publikováno v:
Europe PubMed Central
Sarcomeric mitochondrial creatine kinase (Mib-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli extracts consisted of soluble recombinant Mib-CK in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cac14870820ee4254cc115987e5e539
http://europepmc.org/abstract/med/1471992
http://europepmc.org/abstract/med/1471992