Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Elizabeth J. Pettit"'
Publikováno v:
Biochemical and Biophysical Research Communications. 279:337-340
The rate at which Ca(2+) returns towards the basal concentration is controlled by the action of Ca(2+) pumps, both on the plasma membrane and on organelles within the cytosol. The distribution of Ca(2+) uptake sites within the cytosol was investigate
Publikováno v:
Journal of Cell Science. 111:2209-2215
The role of the transient rise in cytosolic free Ca2+ which occurs during neutrophil adhesion and cell spreading is unclear. In order to establish whether such a Ca2+ signal triggers neutrophil shape change, neutrophils co-loaded with fluo3 and Nitr5
Publikováno v:
Cellular Signalling. 10:49-53
Rapid-time confocal scanning of fluo3-loaded neutrophils revealed that in individual cells there were grossly heterogeneous time intervals between stimulation with either f-met-leu-phe or platelet activating factor (PAF) and the initiation of Ca2+ in
Publikováno v:
Cell Biology International. 21:649-654
The neutrophil represents both the first line ofdefence against infecting microbes and also a po-tential source of tissue destruction during inflam-matory disease. Understanding the signallingprocesses within these cells is thus an importantgoal. T
Publikováno v:
Experimental Cell Research. 234:379-387
The aim of this paper was to establish whether actin polymerization modulated cytosolic Ca2+ storage in human neutrophils. Over the concentration ranges which inhibit actin polymerization, cytochalasins A, B, and D liberated Ca2+ from membrane-bound
Publikováno v:
Journal of Microscopy. 186:275-278
Presenting 3D images produced by confocal optical slicing techniques in a static and easily publishable form can be difficult. Here, we demonstrate the presentation of the data as a single image random dot stereogram (SIRD), which can be viewed as a
Publikováno v:
Journal of Theoretical Biology. 186:1-6
In order to probe the events which couple receptor occupancy to elevation of cytosolic free Ca2+ fast laser scanning of fluo3-loaded neutrophils was used to determine the timing of the initial phase of the Ca2+ response. This approach demonstrated th
Publikováno v:
Biochemical and Biophysical Research Communications. 229:109-113
Confocal laser scanning demonstrated that stimulation of neutrophils with the surface receptor agonist, f-met-leu-phe, resulted in the release of stored Ca2+ from a single site. From reconstructions of neutrophils stained with acridine orange, it was
Publikováno v:
Methods. 9:591-606
Intracellular free Ca2+ plays an important role in the function of neutrophils and many other cell types. In this report, fluorescent techniques for the measurement of intracellular Ca2+ in neutrophils are reviewed. Thus, some commonly used fluoresce
Publikováno v:
Biochemical Journal. 310:445-448
Characterization of early Ca2+ signalling events is crucial for understanding the mechanisms which lead to cell signalling. Rapid confocal laser scanning of Fluo3-loaded neutrophils was used to provide spatially resolved cytosolic free Ca2+ measureme