Zobrazeno 1 - 5
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pro vyhledávání: '"Elizabeth A. Gift"'
Autor:
Elizabeth A. Gift, James C. Weaver
Publikováno v:
Cytometry. 39:243-249
Background: Electroporation is widely used to introduce molecules into cells, but conditions yielding maximal molecular uptake often result in low cell survival. We describe a high throughput method for analyzing populations of culturable cells simul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::954cf4f955b29b5d9f54c4c193116b2e
https://doi.org/10.1002/(sici)1097-0320(20000401)39:4<243::aid-cyto1>3.0.co
https://doi.org/10.1002/(sici)1097-0320(20000401)39:4<243::aid-cyto1>3.0.co
Publikováno v:
Nature Biotechnology. 14:884-887
Isolating hyperproducing cells is important in biotechnology, but these cells usually grow slowly and can be overgrown by poorly producing cells. We describe a new method of isolating slowly growing cells from among rapidly growing cells, which has t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::724fb0fb84e2c33f3036e3cf2c377535
https://doi.org/10.1038/nbt0796-884
https://doi.org/10.1038/nbt0796-884
Publikováno v:
Bioelectrochemistry and Bioenergetics. 39:39-53
Electroporation is well known to depend non-linearly on the magnitude and duration of the change ΔU(t) in transmembrane voltage. In the case of cell suspension experiments, an electric field Ee(t) within the electrolyte causes ΔU(t), which is gover
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::57a575243fdc26df00d14a2328c8dc03
https://doi.org/10.1016/0302-4598(95)05031-0
https://doi.org/10.1016/0302-4598(95)05031-0
Publikováno v:
Electricity and Magnetism in Biology and Medicine ISBN: 9781461372080
The biophysical mechanism governing molecular transport across cell membranes due to external strong electric field pulses is of fundamental interest. Terms such as “breakdown,” “electropermeabilization,” and “electroporation” are widely
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6029a5144fe6cbaf740b335f51647a67
https://doi.org/10.1007/978-1-4615-4867-6_221
https://doi.org/10.1007/978-1-4615-4867-6_221
Autor:
Elizabeth A. Gift, James C. Weaver
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. (1):52-62
Molecular uptake of a charged fluorescent molecule (calcein; 623 Da, z = -4) was quantitatively determined at the single cell level using flow cytometry. Dilutely suspended cells were exposed to one exponential pulse (tau p approximately 300 microsec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12b0b870f0c6651812d3ded31f9b53e5