Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Elisabetta Viani Puglisi"'
Autor:
Betty Ha, Kevin P. Larsen, Jingji Zhang, Ziao Fu, Elizabeth Montabana, Lynnette N. Jackson, Dong-Hua Chen, Elisabetta Viani Puglisi
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Initiation of HIV-1 reverse transcription occurs at the host tRNALys 3, which forms a complex with the 5’ end of the HIV-1 viral RNA and reverse transcriptase (RT). Here, the authors present the 2.8 Å cryo-EM structure of a minimal HIV-1 RT–vRNA
Externí odkaz:
https://doaj.org/article/428ee5420f604c7da4a4e2a37fe10591
Autor:
Albert Tsai, Sotaro Uemura, Magnus Johansson, Elisabetta Viani Puglisi, R. Andrew Marshall, Colin Echeverría Aitken, Jonas Korlach, Måns Ehrenberg, Joseph D. Puglisi
Publikováno v:
Cell Reports, Vol 3, Iss 2, Pp 497-508 (2013)
Inferring antibiotic mechanisms on translation through static structures has been challenging, as biological systems are highly dynamic. Dynamic single-molecule methods are also limited to few simultaneously measurable parameters. We have circumvente
Externí odkaz:
https://doaj.org/article/fd8bba3747bd498782d3adb8bc8bff50
Autor:
Arjun Prabhakar, Natalie Krahn, Jingji Zhang, Oscar Vargas-Rodriguez, Miri Krupkin, Ziao Fu, Francisco J Acosta-Reyes, Xueliang Ge, Junhong Choi, Ana Crnković, Måns Ehrenberg, Elisabetta Viani Puglisi, Dieter Söll, Joseph Puglisi
Publikováno v:
Nucleic acids research. 50(18)
Ribosomes are remarkable in their malleability to accept diverse aminoacyl-tRNA substrates from both the same organism and other organisms or domains of life. This is a critical feature of the ribosome that allows the use of orthogonal translation sy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2b5e92817f0f5cc65397c5a6493a429
https://pubmed.ncbi.nlm.nih.gov/35882385
https://pubmed.ncbi.nlm.nih.gov/35882385
Autor:
Kevin P. Larsen, Betty Ha, Dong-Hua Chen, Elizabeth A. Montabana, Ziao Fu, Jingji Zhang, Elisabetta Viani Puglisi, Lynnette N. Jackson
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Nature Communications
Nature Communications
Reverse transcription of the HIV-1 viral RNA genome (vRNA) is an integral step in virus replication. Upon viral entry, HIV-1 reverse transcriptase (RT) initiates from a host tRNALys3 primer bound to the vRNA genome and is the target of key antivirals
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2005a331a6cfada6ed50b9d9d7204014
https://doaj.org/article/428ee5420f604c7da4a4e2a37fe10591
https://doaj.org/article/428ee5420f604c7da4a4e2a37fe10591
Autor:
Kevin P. Larsen, Aaron T. Coey, Daniel J. Barrero, Elisabetta Viani Puglisi, Junhong Choi, Joseph D. Puglisi
Publikováno v:
Journal of Molecular Biology. 430:5137-5150
The initiation of reverse transcription in human immunodeficiency virus-1 (HIV-1) is a key early step in the virus replication cycle. During this process, the viral enzyme reverse transcriptase (RT) copies the single-stranded viral RNA (vRNA) genome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d37c3bbbe4fd320f78f45ca13382a925
https://doi.org/10.1016/j.jmb.2018.08.029
https://doi.org/10.1016/j.jmb.2018.08.029
Publikováno v:
Current Opinion in Structural Biology
Many viruses, including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and Human Immunodeficiency Virus (HIV), use RNA as their genetic material. How viruses harness RNA structure and RNA-protein interactions to control their replicatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1aaa5e438639bd1c2db91fdf509cd8a3
Autor:
Betty Ha, Kevin P. Larsen, Junhong Choi, Kalli Kappel, Dong-Hua Chen, Elisabetta Viani Puglisi, Lynnette N. Jackson, Jingji Zhang
Publikováno v:
J Mol Biol
A hallmark of the initiation step of HIV-1 reverse transcription, in which viral RNA genome is converted into double-stranded DNA, is that it is slow and non-processive. Biochemical studies have identified specific sites along the viral RNA genomic t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3aa6594d2f632ad2214aee2f3359034
https://pubmed.ncbi.nlm.nih.gov/32512005
https://pubmed.ncbi.nlm.nih.gov/32512005
Autor:
Joseph D. Puglisi, Aaron T. Coey, Yamuna Kalyani Mathiharan, Lauren Madigan, Elisabetta Viani Puglisi, Dong-Hua Chen, Daniel J. Barrero, Georgios Skiniotis, Kevin P. Larsen, Kalli Kappel
Publikováno v:
Nature
Reverse transcription of the HIV-1 RNA genome into double-stranded DNA is a central step in viral infection1 and a common target of antiretroviral drugs2. The reaction is catalysed by viral reverse transcriptase (RT)3,4 that is packaged in an infecti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9ed7d7ab9d40e25289268de8b869d48
https://doi.org/10.1038/s41586-018-0055-9
https://doi.org/10.1038/s41586-018-0055-9
Publikováno v:
Cold Spring Harb Perspect Biol
Recent advances in structural biology methods have enabled a surge in the number of RNA and RNA-protein assembly structures available at atomic or near-atomic resolution. These complexes are often trapped in discrete conformational states that exist
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9798681d0aedf6679e1a5ee03f2752d9
https://europepmc.org/articles/PMC6601459/
https://europepmc.org/articles/PMC6601459/
Publikováno v:
RNA. 22:1689-1698
Reverse transcription is a key process in the early steps of HIV infection. This process initiates within a specific complex formed by the 5′ UTR of the HIV genomic RNA (vRNA) and a host primer tRNALys3. Using nuclear magnetic resonance (NMR) spect
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6efe302350fff64e967f64628ed5fe0
https://doi.org/10.1261/rna.056804.116
https://doi.org/10.1261/rna.056804.116