Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Elisabeth, Petfalski"'
Autor:
Maxime Duval, Carlo Yague-Sanz, Tomasz W. Turowski, Elisabeth Petfalski, David Tollervey, François Bachand
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract Transcription by RNA polymerase I (RNAPI) represents most of the transcriptional activity in eukaryotic cells and is associated with the production of mature ribosomal RNA (rRNA). As several rRNA maturation steps are coupled to RNAPI transcr
Externí odkaz:
https://doaj.org/article/319fde815ac84a9cb9fc2cc527d6bb29
Autor:
Vadim Shchepachev, Stefan Bresson, Christos Spanos, Elisabeth Petfalski, Lutz Fischer, Juri Rappsilber, David Tollervey
Publikováno v:
Molecular Systems Biology, Vol 15, Iss 4, Pp 1-23 (2019)
Abstract The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)‐associated proteins. However, most cellular transcripts are not polyadenylated. We therefore developed total RNA‐associated p
Externí odkaz:
https://doaj.org/article/f7f7fda2b3e440748553be9aec71562f
Autor:
Laura Milligan, Vân A Huynh‐Thu, Clémentine Delan‐Forino, Alex Tuck, Elisabeth Petfalski, Rodrigo Lombraña, Guido Sanguinetti, Grzegorz Kudla, David Tollervey
Publikováno v:
Molecular Systems Biology, Vol 12, Iss 6, Pp 1-15 (2016)
Abstract Reversible modification of the RNAPII C‐terminal domain links transcription with RNA processing and surveillance activities. To better understand this, we mapped the location of RNAPII carrying the five types of CTD phosphorylation on the
Externí odkaz:
https://doaj.org/article/0409b0dae53f4906ad18cf6fa2f647a8
Autor:
Mirjam Hunziker, Jonas Barandun, Elisabeth Petfalski, Dongyan Tan, Clémentine Delan-Forino, Kelly R. Molloy, Kelly H. Kim, Hywel Dunn-Davies, Yi Shi, Malik Chaker-Margot, Brian T. Chait, Thomas Walz, David Tollervey, Sebastian Klinge
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
Eukaryotic ribosome biogenesis involves a large number of maturations factors which are responsible for the stepwise assembly of the ribosomal subunits. Here the authors use an array of biochemical and structural biology methods to investigate the fu
Externí odkaz:
https://doaj.org/article/8f1bcb93f5c5482ca631db107b522b3f
Autor:
David Tollervey, Christos Spanos, Stefan Bresson, Juri Rappsilber, Vadim Shchepachev, Lutz Fischer, Elisabeth Petfalski
Publikováno v:
Shchepachev, V, Bresson, S, Spanos, C, Petfalski, E, Fischer, L, Rappsilber, J & Tollervey, D 2019, ' Defining the RNA interactome by total RNA-associated protein purification ', Molecular Systems Biology, vol. 15, no. 4, e8689 . https://doi.org/10.15252/msb.20188689
UV crosslinking can be used to identify precise RNA targets for individual proteins, transcriptome-wide. We sought to develop a technique to generate reciprocal data, identifying precise sites of RNA-binding proteome-wide. The resulting technique, to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24c04a152753969d35f42ba127c93a43
https://www.pure.ed.ac.uk/ws/files/82579560/Defining_the_RNA_interactome_PUB_OA_CC_BY_e8689.full.pdf
https://www.pure.ed.ac.uk/ws/files/82579560/Defining_the_RNA_interactome_PUB_OA_CC_BY_e8689.full.pdf
Autor:
Thomas Walz, Kelly H. Kim, Hywel Dunn-Davies, Clémentine Delan-Forino, Mirjam Hunziker, Jonas Barandun, Elisabeth Petfalski, Brian T. Chait, Sebastian Klinge, Kelly R. Molloy, Malik Chaker-Margot, Dongyan Tan, David Tollervey, Yi Shi
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
Hunziker, M, Barandun, J, Petfalski, E, Tan, D, Delan-Forino, C, Molloy, K R, Kim, K H, Dunn-Davies, H, Shi, Y, Chaker-Margot, M, Chait, B T, Walz, T, Tollervey, D & Klinge, S 2016, ' UtpA and UtpB chaperone nascent pre-ribosomal RNA and U3 snoRNA to initiate eukaryotic ribosome assembly ', Nature Communications, vol. 7, 12090 . https://doi.org/10.1038/ncomms12090
Nature Communications
Hunziker, M, Barandun, J, Petfalski, E, Tan, D, Delan-Forino, C, Molloy, K R, Kim, K H, Dunn-Davies, H, Shi, Y, Chaker-Margot, M, Chait, B T, Walz, T, Tollervey, D & Klinge, S 2016, ' UtpA and UtpB chaperone nascent pre-ribosomal RNA and U3 snoRNA to initiate eukaryotic ribosome assembly ', Nature Communications, vol. 7, 12090 . https://doi.org/10.1038/ncomms12090
Nature Communications
Early eukaryotic ribosome biogenesis involves large multi-protein complexes, which co-transcriptionally associate with pre-ribosomal RNA to form the small subunit processome. The precise mechanisms by which two of the largest multi-protein complexes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8af490fef8002f02a43522ce710249b0
https://doaj.org/article/8f1bcb93f5c5482ca631db107b522b3f
https://doaj.org/article/8f1bcb93f5c5482ca631db107b522b3f
Autor:
Benjamin D. Goddard, Aleksandra Helwak, Elisabeth Petfalski, Tomasz W. Turowski, David Tollervey, Sarah L. French
Publikováno v:
Molecular Cell
Turowski, T, Petfalski, E, Goddard, B D, French, S L, Helwak, A & Tollervey, D 2020, ' Nascent transcript folding plays a major role in determining RNA polymerase elongation rates ', Molecular Cell, vol. 79, no. 3, pp. 488-503.e11 . https://doi.org/10.1016/j.molcel.2020.06.002
Turowski, T, Petfalski, E, Goddard, B D, French, S L, Helwak, A & Tollervey, D 2020, ' Nascent transcript folding plays a major role in determining RNA polymerase elongation rates ', Molecular Cell, vol. 79, no. 3, pp. 488-503.e11 . https://doi.org/10.1016/j.molcel.2020.06.002
Summary Transcription elongation rates influence RNA processing, but sequence-specific regulation is poorly understood. We addressed this in vivo, analyzing RNAPI in S. cerevisiae. Mapping RNAPI by Miller chromatin spreads or UV crosslinking revealed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a4ebe0de430ec8a2e5befdc70a6e39e
https://doi.org/10.1016/j.molcel.2020.06.002
https://doi.org/10.1016/j.molcel.2020.06.002
Autor:
Helena Santos-Rosa, Samuel Robson, Gonzalo Millán-Zambrano, Elisabeth Petfalski, David Tollervey, Camille Sayou, Jonathan Houseley, Tony Kouzarides
Publikováno v:
Molecular and Cellular Biology
Sayou, C, Millan-Zambrano, G, Santos-Rosa, H, Petfalski, E, Robson, S, Houseley, J, Kouzarides, T & Tollervey, D 2017, ' RNA binding by the histone methyltransferases Set1 and Set2 ', Molecular and Cellular Biology, vol. 37, no. 14, e00165-17 . https://doi.org/10.1128/MCB.00165-17
Sayou, C, Millan-Zambrano, G, Santos-Rosa, H, Petfalski, E, Robson, S, Houseley, J, Kouzarides, T & Tollervey, D 2017, ' RNA binding by the histone methyltransferases Set1 and Set2 ', Molecular and Cellular Biology, vol. 37, no. 14, e00165-17 . https://doi.org/10.1128/MCB.00165-17
Histone methylation at H3K4 and H3K36 is commonly associated with genes actively transcribed by RNA polymerase II (RNAPII) and is catalyzed by Saccharomyces cerevisiae Set1 and Set2, respectively. Here we report that both methyltransferases can be UV
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e98bb2551938e87c4fad50ce25fd772
http://europepmc.org/articles/PMC5492175
http://europepmc.org/articles/PMC5492175
Autor:
Laura, Milligan, Vân A, Huynh-Thu, Clémentine, Delan-Forino, Alex, Tuck, Elisabeth, Petfalski, Rodrigo, Lombraña, Guido, Sanguinetti, Grzegorz, Kudla, David, Tollervey
Publikováno v:
Molecular Systems Biology
Reversible modification of the RNAPII C‐terminal domain links transcription with RNA processing and surveillance activities. To better understand this, we mapped the location of RNAPII carrying the five types of CTD phosphorylation on the RNA trans
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::decec78c5c91370e7a6e111041a1347e
http://hdl.handle.net/20.500.11767/117343
http://hdl.handle.net/20.500.11767/117343
Publikováno v:
The EMBO Journal. 30:4006-4019
The 5′-exonuclease Rat1 degrades pre-rRNA spacer fragments and processes the 5′-ends of the 5.8S and 25S rRNAs. UV crosslinking revealed multiple Rat1-binding sites across the pre-rRNA, consistent with its known functions. The major 5.8S 5′-end
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a331e8f47dbd5f6adb06722d281589d1
https://doi.org/10.1038/emboj.2011.256
https://doi.org/10.1038/emboj.2011.256