Structural dynamics of DNA strand break sensing by PARP-1 at a single-molecule level

Autor: Anna Sefer, Eleni Kallis, Tobias Eilert, Carlheinz Röcker, Olga Kolesnikova, David Neuhaus, Sebastian Eustermann, Jens Michaelis

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)

Sm FRET studies of PARP-1 recognition of DNA lesion show that binding of F1F2 fragment results in a kinked DNA, while binding of F2 fragment alone yields an intermediate state. This dynamic equilibrium of states is influenced by inhibitors.

Externí odkaz: https://doaj.org/article/642a60ff46824c648ef3c679a670721e

Single-molecule nucleosome remodeling by INO80 and effects of histone tails

Autor: Eleni Kallis, Mara Guariento, Kevin Schall, Jens Michaelis, Marianne Schwarz, Sebastian Eustermann, Karl-Peter Hopfner, Manuela Moldt

Publikováno v: FEBS Letters. 592:318-331

Genome maintenance and integrity requires continuous alterations of the compaction state of the chromatin structure. Chromatin remodelers, among others the INO80 complex, help organize chromatin by repositioning, reshaping, or evicting nucleosomes. W

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04ec0052c12c9d880946f3b487bd38d0
https://doi.org/10.1002/1873-3468.12973

Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study

Autor: Taekjip Ha, Tim Schröder, Philip Tinnefeld, Marcia Levitus, Benjamin Schuler, Christian G. Hübner, Björn Hellenkamp, Christian A. Hanke, Markus Götz, Enrico Gratton, Johannes Hohlbein, Benjamin Ambrose, Sonja Schmid, Jens Michaelis, Anders Barth, Soheila Rezaei Adariani, Mark E. Bowen, Carel Fijen, Eleni Kallis, Edward A. Lemke, Jelle Hendrix, Victoria Birkedal, Thuy T.M. Ngo, Ralf Kühnemuth, Bettina Wünsch, Lasse L. Hildebrandt, Claus A. M. Seidel, Timothy D. Craggs, Henning Seidel, Georg Krainer, Michael Schlierf, Swati Tyagi, Hugo Sanabria, Mikayel Aznauryan, Niels Vandenberk, Giorgos Gouridis, Verena Hirschfeld, Daniel Nettels, Jae-Yeol Kim, Inna S. Yanez-Orozco, Pengyu Hao, Achillefs N. Kapanidis, Brié Levesque, Thorsten Hugel, James J. McCann, Nam Ki Lee, Oleg Opanasyuk, Andreas Hartmann, Johann Thurn, Hongtao Chen, Tobias Eilert, Lisa Streit, Don C. Lamb, Carlheinz Röcker, Ruoyi Qiu, Keith Weninger, Christian Gebhardt, Thorben Cordes, Nicole C. Robb, Nikolaus Naredi-Rainer, Andrés Manuel Vera, Boyang Hua, Olga Doroshenko

Publikováno v: Nature Methods, 15(9), 669-676. Nature Publishing Group
Nature methods
Nature Methods 15 (2018) 9
Hellenkamp, Björn; Schmid, Sonja; Doroshenko, Olga; Opanasyuk, Oleg; Kühnemuth, Ralf; Rezaei Adariani, Soheila; et al.(2018). Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study.. Nature methods, 15(9), 669-676. doi: 10.1038/s41592-018-0085-0. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/82n6186z
Nature Methods
Hellenkamp, B, Schmid, S, Doroshenko, O, Opanasyuk, O, Kuehnemuth, R, Adariani, S R, Ambrose, B, Aznauryan, M, Barth, A, Birkedal, V, Bowen, M E, Chen, H, Cordes, T, Eilert, T, Fijen, C, Gebhardt, C, Goetz, M, Gouridis, G, Gratton, E, Ha, T, Hao, P, Hanke, C A, Hartmann, A, Hendrix, J, Hildebrandt, L L, Hirschfeld, V, Hohlbein, J, Hua, B, Huebner, C G, Kallis, E, Kapanidis, A N, Kim, J-Y, Krainer, G, Lamb, D C, Lee, N K, Lemke, E A, Levesque, B, Levitus, M, McCann, J J, Naredi-Rainer, N, Nettels, D, Thuy Ngo, T, Qiu, R, Robb, N C, Roecker, C, Sanabria, H, Schlierf, M, Schroeder, T, Schuler, B, Seidel, H, Streit, L, Thurn, J, Tinnefeld, P, Tyagi, S, Vandenberk, N, Vera, A M, Weninger, K R, Wunsch, B, Yanez-Orozco, I S, Michaelis, J, Seidel, C A M, Craggs, T D & Hugel, T 2018, ' Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study ', Nature Methods, vol. 15, no. 9, pp. 669-676 . https://doi.org/10.1038/s41592-018-0085-0
Nature Methods, 15(9), 669-676
Nature Methods (online), 15(9)

Single-molecule Forster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibili

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72edbb856467f1a0f8cea053565b29e1
https://research.rug.nl/en/publications/6b30271d-1e88-40c6-a10a-544c2685cb93

Acetylation of histone H3 at lysine 64 regulates nucleosome dynamics and facilitates transcription

Autor: Emilie Montellier, Salim Kacem, Andrew J. Bannister, Vincenzo Di Cerbo, Robert Feil, Florian Richter, Jens Michaelis, Philipp Tropberger, Eleni Kallis, Gerhard Mittler, Angelika Feldmann, Dirk Schuebeler, Leslie Hoerner, Sylvain Daujat, Fabio Mohn, Saadi Khochbin, Tom Owen-Hughes, Monika Holzner, Daniel P. Ryan, Robert Schneider

Publikováno v: eLife
eLife, eLife Sciences Publication, 2014, 3, pp.e01632. ⟨10.7554/eLife.01632⟩
eLife, Vol 3 (2014)

Post-translational modifications of proteins have emerged as a major mechanism for regulating gene expression. However, our understanding of how histone modifications directly affect chromatin function remains limited. In this study, we investigate a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd94a999ae865c3f0cf84922c56addd7