Catalytic Knockdown of miR-21 by Artificial Ribonuclease: Biological Performance in Tumor Model

Autor: Olga A. Patutina, Svetlana K. Miroshnichenko, Nadezhda L. Mironova, Aleksandra V. Sen’kova, Elena V. Bichenkova, David J. Clarke, Valentin V. Vlassov, Marina A. Zenkova

Publikováno v: Frontiers in Pharmacology, Vol 10 (2019)

Control of the expression of oncogenic small non-coding RNAs, notably microRNAs (miRNAs), is an attractive therapeutic approach. We report a design platform for catalytic knockdown of miRNA targets with artificial, sequence-specific ribonucleases. mi

Externí odkaz: https://doaj.org/article/f4b56814e4af47f7b059a8be3d18d8f9

'Bind, cleave and leave': multiple turnover catalysis of RNA cleavage by bulge-loop inducing supramolecular conjugates

Autor: Bahareh Amirloo, Yaroslav Staroseletz, Sameen Yousaf, David J Clarke, Tom Brown, Harmesh Aojula, Marina A Zenkova, Elena V Bichenkova

Publikováno v: Nucleic Acids Research
Amirloo, B, Staroseletz, Y, Yousaf, S, Clarke, D J, Brown, T, Aojula, H, Zenkova, M A & Bichenkova, E V 2021, ' “Bind, cleave and leave”: multiple turnover catalysis of RNA cleavage by bulge–loop inducing supramolecular conjugates ', Nucleic acids research . https://doi.org/10.1093/nar/gkab1273

Antisense sequence-specific knockdown of pathogenic RNA offers opportunities to find new solutions for therapeutic treatments. However, to gain a desired therapeutic effect, the multiple turnover catalysis is critical to inactivate many copies of eme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f7b808fe6bedc0a0aab05633989d108
https://pubmed.ncbi.nlm.nih.gov/34967410

Strict conformational demands of RNA cleavage in bulge-loops created by peptidyl-oligonucleotide conjugates

Autor: Marina A. Zenkova, David Clarke, Tom Brown, Bahareh Amirloo, Kepa K. Burusco, Alexander A. Lomzov, Aled Williams, Elena V. Bichenkova, Yaroslav Staroseletz

Publikováno v: Staroseletz, Y, Amirloo, B, Williams, A, Lomzov, A, Burusco, K, Clarke, D J, Brown, T, Zenkova, M A & Bichenkova, E 2020, ' Strict conformational demands of RNA cleavage in bulge-loops created by peptidyl-oligonucleotide conjugates ', Nucleic acids research, vol. 48, no. 19, pp. 10662-10679 . https://doi.org/10.1093/nar/gkaa780
University of Manchester-PURE
Staroseletz, Y, Amirloo, B, Williams, A, Lomzov, A, Burusco-Goni, K, Clarke, D, Brown, T, Zenkova, M A & Bichenkova, E 2020, ' Strict conformational demands of RNA cleavage in bulgeloops created by peptidyl-oligonucleotide conjugates ', Nucleic Acids Res, vol. 48, no. 19, pp. 10662-10679 . < https://doi.org/10.1093/nar/gkaa780 >
Nucleic Acids Research

Potent knockdown of pathogenic RNA in vivo is an urgent health need unmet by both small-molecule and biologic drugs. ‘Smart’ supramolecular assembly of catalysts offers precise recognition and potent destruction of targeted RNA, hitherto not foun

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3370e00e689f01039fccc16fa1d0b0f
https://www.research.manchester.ac.uk/portal/en/publications/strict-conformational-demands-of-rna-cleavage-in-bulgeloops-created-by-peptidyloligonucleotide-conjugates(faa0502c-dbe3-4266-83ca-0e4fa9f90b67).html

RNA knockdown by synthetic peptidyl-oligonucleotide ribonucleases: behavior of recognition and cleavage elements under physiological conditions

Autor: Antonina A. Fedorova, M. B. Gebrezgiabher, Waleed Abdelhalim Yousef Zalloum, Elena V. Bichenkova, Steven M. Miles, Marina A. Zenkova, David Clarke

Publikováno v: Gebrezgiabher, M, Zalloum, W A Y, Clarke, D, Miles, S, Fedorova, A A, Zenkova, M & Bichenkova, E 2020, ' RNA knockdown by synthetic peptidyl-oligonucleotide ribonucleases : behavior of recognition and cleavage elements under physiological conditions ', Journal of Biomolecular Structure and Dynamics . https://doi.org/10.1080/07391102.2020.1751711

Sequence-specific protein-based ribonucleases are not found in nature. Absolute sequence selectivity in RNA cleavage in vivo normally requires multi-component complexes that recruit a guide RNA or DNA for target recognition and a protein–RNA assemb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c667e5842de77ee011ed9120119186db
https://pubmed.ncbi.nlm.nih.gov/32248755

Sequence-Specific Detection of Unlabeled Nucleic Acid Biomarkers Using a 'One-Pot' 3D Molecular Sensor

Autor: Sameen, Yousaf, Patrick J S, King, Aline F, Miller, Alberto, Saiani, David J, Clarke, Linda T, Trivoluzzi, Harmesh S, Aojula, Elena V, Bichenkova

Publikováno v: Analytical Chemistry

DNA and RNA biomarkers have not progressed beyond the automated specialized clinic due to failure in the reproducibility necessary to standardize robust and rapid nucleic acid detection at the point of care, where health outcomes can be most improved

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::da58403a229c696185382757aafde6fc
https://pubmed.ncbi.nlm.nih.gov/31246004