Chromatin accessibility profiling of targeted cell populations with laser capture microdissection coupled to ATAC-seq

Autor: Caterina Carraro, Lorenzo Bonaguro, Rachana Srinivasa, Martina van Uelft, Victoria Isakzai, Jonas Schulte-Schrepping, Prerna Gambhir, Tarek Elmzzahi, Jessica V. Montgomery, Hannah Hayer, Yuanfang Li, Heidi Theis, Michael Kraut, Krishnaa T. Mahbubani, Anna C. Aschenbrenner, Ireen König, Eugenio Fava, Hans-Ulrich Fried, Elena De Domenico, Marc Beyer, Adem Saglam, Joachim L. Schultze

Publikováno v: Cell Reports: Methods, Vol 3, Iss 10, Pp 100598- (2023)

Summary: Spatially resolved omics technologies reveal context-dependent cellular regulatory networks in tissues of interest. Beyond transcriptome analysis, information on epigenetic traits and chromatin accessibility can provide further insights on g

Externí odkaz: https://doaj.org/article/ea2a676cee80406e904209f8d76f1bfe

Analysis of the early response to spinal cord injury identified a key role for mTORC1 signaling in the activation of neural stem progenitor cells

Autor: Johany Peñailillo, Miriam Palacios, Constanza Mounieres, Rosana Muñoz, Paula G. Slater, Elena De Domenico, Ilya Patrushev, Mike Gilchrist, Juan Larraín

Publikováno v: npj Regenerative Medicine, Vol 6, Iss 1, Pp 1-15 (2021)

Abstract Xenopus laevis are able to regenerate the spinal cord during larvae stages through the activation of neural stem progenitor cells (NSPCs). Here we use high-resolution expression profiling to characterize the early transcriptome changes induc

Externí odkaz: https://doaj.org/article/8e9c4b3dffcb4bd898001e36e05bb8fc

Disease severity-specific neutrophil signatures in blood transcriptomes stratify COVID-19 patients

Autor: Anna C. Aschenbrenner, Maria Mouktaroudi, Benjamin Krämer, Marie Oestreich, Nikolaos Antonakos, Melanie Nuesch-Germano, Konstantina Gkizeli, Lorenzo Bonaguro, Nico Reusch, Kevin Baßler, Maria Saridaki, Rainer Knoll, Tal Pecht, Theodore S. Kapellos, Sarandia Doulou, Charlotte Kröger, Miriam Herbert, Lisa Holsten, Arik Horne, Ioanna D. Gemünd, Nikoletta Rovina, Shobhit Agrawal, Kilian Dahm, Martina van Uelft, Anna Drews, Lena Lenkeit, Niklas Bruse, Jelle Gerretsen, Jannik Gierlich, Matthias Becker, Kristian Händler, Michael Kraut, Heidi Theis, Simachew Mengiste, Elena De Domenico, Jonas Schulte-Schrepping, Lea Seep, Jan Raabe, Christoph Hoffmeister, Michael ToVinh, Verena Keitel, Gereon Rieke, Valentina Talevi, Dirk Skowasch, N. Ahmad Aziz, Peter Pickkers, Frank L. van de Veerdonk, Mihai G. Netea, Joachim L. Schultze, Matthijs Kox, Monique M. B. Breteler, Jacob Nattermann, Antonia Koutsoukou, Evangelos J. Giamarellos-Bourboulis, Thomas Ulas, German COVID-19 Omics Initiative (DeCOI)

Publikováno v: Genome Medicine, Vol 13, Iss 1, Pp 1-25 (2021)

Abstract Background The SARS-CoV-2 pandemic is currently leading to increasing numbers of COVID-19 patients all over the world. Clinical presentations range from asymptomatic, mild respiratory tract infection, to severe cases with acute respiratory d

Externí odkaz: https://doaj.org/article/00693afe88234ab7b97afcce182c0fb0

Optimized workflow for single-cell transcriptomics on infectious diseases including COVID-19

Autor: Elena De Domenico, Lorenzo Bonaguro, Jonas Schulte-Schrepping, Matthias Becker, Kristian Händler, Joachim L. Schultze

Publikováno v: STAR Protocols, Vol 1, Iss 3, Pp 100233- (2020)

Summary: In December 2019, a new coronavirus, SARS-CoV-2, which causes the respiratory illness that led to the COVID-19 pandemic, was reported. In the face of such a new pathogen, special precautions must be taken to examine potentially infectious ma

Externí odkaz: https://doaj.org/article/67e835862f694defab9eab92ef77aa6a

Analysis of the early response to spinal cord injury identified a key role for mTORC1 signaling in the activation of neural stem progenitor cells

Autor: Paula G. Slater, Michael J. Gilchrist, Ilya Patrushev, Miriam Palacios, Rosana Muñoz, Elena De Domenico, Johany Peñailillo, Constanza Mounieres, Juan Larraín

Publikováno v: npj Regenerative Medicine, Vol 6, Iss 1, Pp 1-15 (2021)
NPJ Regenerative Medicine
npj regenerative medicine 6(1), 68 (2021). doi:10.1038/s41536-021-00179-3

Xenopus laevis are able to regenerate the spinal cord during larvae stages through the activation of neural stem progenitor cells (NSPCs). Here we use high-resolution expression profiling to characterize the early transcriptome changes induced after

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b19970186789f00662c84a6f8803bf6
https://doaj.org/article/8e9c4b3dffcb4bd898001e36e05bb8fc