Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Elena Cardellini"'
Publikováno v:
Peptides. 25:191-197
Synthetic peptides containing a phosphorylation site for protein kinase CK2 were used to investigate their binding properties to other peptides/proteins. The aim of this work was to find an efficient procedure to search for these peptide/protein liga
Autor:
Gian Luigi Gianfranceschi, Elena Cardellini, Aldo Santucci, Lorena Urbanelli, Stefania Cinelli, Giuseppe Onori
Publikováno v:
Molecular Biology Reports. 27:175-180
The thermal denaturation of calf thymus total chromatin and of fractions enriched in heterochromatin or euchromatin, has been investigated by differential scanning calorimetry and compared to that of calf thymus DNA and DNA-histone complexes. In our
Publikováno v:
Molecular Biology Reports. 26:255-259
Small acidic peptides involved in gene expression have been isolated from prokaryotic and eukaryotic cells. Synthetic peptides, designed on the basis of native peptides characteristics, show a biological activity similar to that of native peptides in
Publikováno v:
Molecular Biology Reports. 26:249-253
Low-molecular-weight peptides involved in gene expression and cell growth have been isolated from DNA preparation from eukaryotic cells. After phosphorylation with protein kinase CKII (pCKII) these peptides are able to bind to DNA in presence of diva
Publikováno v:
Molecular and Cellular Biochemistry. 178:393-396
E. Coli RNA polymerase was phosphorylated with protein kinase CKII and allowed to bind to pBR322. After digestion of the RNA polymerase-pBR322 complex with proteinase K, the phosphopeptides that remained bound to DNA were extracted and analyzed. Thes
Publikováno v:
Biological Chemistry Hoppe-Seyler. 375:255-260
Topoisomerase I purified from HeLa cells was phosphorylated in vitro with protein kinase NII (pkNII) purified from calf thymus: this phosphorylation was inhibited by heparin. A peptide containing a sequence corresponding to a putative pkNII phosphory
Autor:
Francesco Chillemi, G. L. Gianfranceschi, D. Boari, Domenico Amici, Massimo Bramucci, Egon Durban, Elena Cardellini, Antonino Miano, Lugaro G
Publikováno v:
FEBS Letters. 291:67-70
The pentapeptide pyroGlu-Ala-Glu-Ser-Asn has been synthetized and phosphorylated in vitro at level of serine by protein kinase NII isolated from calf thymus chromatin. It is noteworthy that the calf thymus kinase NII shows a remarkable affinity for t
Autor:
Elena Cardellini, Gian Luigi Gianfranceschi, Francesco Chillemi, Valeria Marsili, Lugaro G, L. De Angelis, F. Giavarini, Loretta Mancinelli
Publikováno v:
Biological chemistry. 380(1)
Small acidic peptides have been isolated from biological fluids (blood and seminal plasma) and from chromatin of several tissues. Their biological activity is related to the control of cell growth and gene expression. This work is an approach to the
Autor:
Gina Piccinini, Ennio Maccari, Elena Cardellini, Antonino Miano, Massimo Bramucci, Domenico Amici, Gian Luigi Gianfranceschi
Publikováno v:
The International journal of biochemistry. 25(7)
1. 1. Highly purified DNA from calf thymus was phosphorylated with protein kinase NII. 2. 2. Digestion with proteinase K of this DNA demonstrates proteins as phosphorylated component. 3. 3. Gel filtration chromatography on Bio-Gel A-0.5m gel column s
Publikováno v:
Molecular immunology. 28(4-5)
Topoisomerase cDNA and various fragments thereof generated by the DNA polymerase chain reaction were cloned into plasmid expression vectors (pET series) and the expressed polypeptides were probed with scleroderma sera from seven different patients im