A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function

Autor: Christopher J. Perry, Eleanor C. Warren, Joseph L. Damstra-Oddy, Claire Storey, Lisa M. Francione, Sarah J. Annesley, Paul R. Fisher, Annette Müller-Taubenberger, Robin S.B. Williams

Publikováno v: Biochemistry and Biophysics Reports, Vol 22, Iss , Pp - (2020)

Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fl

Externí odkaz: https://doaj.org/article/6a821dd9f14e40598a28665964f99374

All You Need Is Fats—for Seizure Control: Using Amoeba to Advance Epilepsy Research

Autor: Eleanor C. Warren, Matthew C. Walker, Robin S. B. Williams

Publikováno v: Frontiers in Cellular Neuroscience, Vol 12 (2018)

Since the original report of seizure control through starvation in the 1920s, the ketogenic diet has been considered an energy-related therapy. The diet was assumed to be functioning through the effect of reduced carbohydrate intake regulating cellul

Externí odkaz: https://doaj.org/article/ee325fa55f3d4ec5a6e9b4f1e81a4dd6

Phytocannabinoid‐dependent mTORC1 regulation is dependent upon inositol polyphosphate multikinase activity

Autor: Eleanor C. Warren, Joseph Damstra-Oddy, Lisa Costelloe, John-Mark K. Fitzpatrick, Yann Desfougères, Christopher J. Perry, Robin S.B. Williams, Adolfo Saiardi, Judith Schaf, Eric J. Downer, William H Hind

Publikováno v: British Journal of Pharmacology. 178:1149-1163

Background and purpose Cannabidiol (CBD) has been shown to differentially regulate the mechanistic target of rapamycin complex 1 (mTORC1) in preclinical models of disease, where it reduces activity in models of epilepsies and cancer and increases it

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d778dc0e9a75c13f1974403071e6d1f
https://doi.org/10.1111/bph.15351

Decanoic acid inhibits mTORC1 activity independent of glucose and insulin signaling

Autor: Robin S.B. Williams, Eleonora Lugarà, Joseph Damstra-Oddy, Eleanor C. Warren, Vivi M. Heine, Judith Schaf, Stephanie Dooves, Matthew C. Walker

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 117(38), 23617-23625. National Acad Sciences
Proceedings of the National Academy of Sciences of the United States of America, 117(38), 23617-23625. National Academy of Sciences
Warren, E C, Dooves, S, Lugarà, E, Damstra-Oddy, J, Schaf, J, Heine, V M, Walker, M C & Williams, R S B 2020, ' Decanoic acid inhibits mTORC1 activity independent of glucose and insulin signaling ', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 38, pp. 23617-23625 . https://doi.org/10.1073/pnas.2008980117
Proceedings of the National Academy of Sciences of the United States of America

Significance The mTORC1 complex provides a critical role in cell function, regulating a variety of processes including growth and autophagy. mTORC1 signaling is hyperactivated in a range of common diseases including cancer, epilepsy, and neurodegener

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b19e3ae2d3ba9f9265e903dc3f80f665
https://research.vu.nl/en/publications/d95d1234-573b-4308-8a86-e833e7ba0ea7

A new mechanism for Cannabidiol in regulating the one-carbon cycle and methionine levels in Dictyostelium and in mammalian epilepsy models

Autor: Nicholas D. E. Greene, Joseph Damstra-Oddy, Joanna Boberska, Robert J. Harvey, Paul Finch, Amy Joy Baldwin, Christopher J. Perry, Christopher R. L. Thompson, Fabiana Piscitelli, Adrian J. Harwood, Kit-Yi Leung, Eleanor C. Warren, Robin S.B. Williams, Benjamin J. Whalley, Balint Stewart, Alister J. McNeish, Annette Müller-Taubenberger, Claire M. Williams, Devdutt Sharma, Sarah Glyn, Sandrine P. Claus, Pabitra Hriday Patra

Publikováno v: British journal of pharmacology (Online) (2020).
info:cnr-pdr/source/autori:Perry CJ, Finch P, Müller-Taubenberger A, Leung KY, Warren EC, Damstra-Oddy J, Sharma D, Patra PH, Glyn S, Boberska J, Stewart B, Baldwin A, Piscitelli F, Harvey RJ, Harwood A, Thompson C, Claus S, Greene NDE, McNeish A, Williams CM, Whalley BJ, Williams RSB./titolo:A new mechanism for Cannabidiol in regulating the one-carbon cycle and methionine levels in Dictyostelium and in mammalian epilepsy models./doi:/rivista:British journal of pharmacology (Online)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume
Perry, C J, Finch, P, Müller-Taubenberger, A, Leung, K-Y, Warren, E C, Damstra-Oddy, J, Sharma, D, Patra, P H, Glyn, S, Boberska, J, Stewart, B, Baldwin, A, Piscitelli, F, Harvey, R J, Harwood, A, Thompson, C, Claus, S, Greene, N D E, McNeish, A, Williams, C M, Whalley, B J & Williams, R S B 2019, ' A new mechanism for Cannabidiol in regulating the one-carbon cycle and methionine levels in Dictyostelium and in mammalian epilepsy models ', British Journal of Pharmacology . https://doi.org/10.1111/bph.14892
British Journal of Pharmacology

Background and Purpose: Epidiolex, a form of highly purified cannabidiol (CBD) derived from Cannabis plants has demonstrated seizure control activity in patients with Dravet syndrome, without a fully elucidated mechanism of action. We have employed a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3a90729a06a68fac2f2a4686eb515dd
https://publications.cnr.it/doc/411516