Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Elana Swartzman"'
Autor:
S. Cribbes, H. Stoop, Astrid Ferlinz, J. W. Oosterhuis, Elana Swartzman, Ad J. M. Gillis, Mark Shannon, Katharina Biermann, R. J. H. L. M. Van Gurp, Leendert H. J. Looijenga
Publikováno v:
International Journal of Andrology. 34:e160-e174
OCT3/4, NANOG, SOX2 and, most recently, LIN28 have been identified as key regulators of pluripotency in mammalian embryonic and induced stem cells, and are proven to be crucial for generation of the mouse germ-cell lineage. These factors are a hallma
Autor:
Julie Kuykendall, Chad Mooney, David Ruff, Rouying Tan, Elana Swartzman, Tina Settineri, Pauline Lieu, Shiaw-Min Chen, Levente Egry, Mark Shannon, Eric Wei
Publikováno v:
Methods. 50:S23-S26
The correlation of gene and protein expression changes in biological systems has been hampered by the need for separate sample handling and analysis platforms for nucleic acids and proteins. In contrast to the simple, rapid, and flexible workflow of
Autor:
Edward M. Goldberg, Iwan Gunawan, Christopher Smith, Kenton Lohman, Julia Mellentin-Michelotti, Pau-Miau Yuan, Bala Manian, Elana Swartzman, Lolita Evangelista, Sheri Miraglia
Publikováno v:
SLAS Discovery. 4:193-204
High throughput drug screening has become a critical component of the drug discovery process. The screening of libraries containing hundreds of thousands of compounds has resulted in a requirement for assays and instrumentation that are amenable to n
Autor:
Julia Mellentin-Michelotti, Sheri Miraglia, Lolita Evangelista, Pau-Miau Yuan, Elana Swartzman
Publikováno v:
Analytical Biochemistry. 271:143-151
We have developed a simple, homogeneous bead-based immunoassay for use with fluorometric microvolume assay technology (FMAT). The FLISA (fluorescence-linked immunosorbent assay) can be easily adapted from existing immunoassays, is comparable to tradi
Publikováno v:
Molecular Microbiology. 19:767-775
Analysis of Vibrio harveyi dark autoinducer mutants has demonstrated that the level of LuxR was much lower than that observed in wild-type cells. Complementation with luxR fully restored luminescence suggesting that the lux autoinducer may control ex
Autor:
Elana Swartzman, Edward A. Meighen
Publikováno v:
Biochemistry and Cell Biology. 70:698-702
RNA polymerase was purified from Vibrio harveyi and found to contain polypeptides (β,β′, α, and σ) closely corresponding to those of the Escherichia coli enzyme. In vitro transcription studies using V. harveyi and E. coli RNA polymerase demonst
Autor:
Jennifer Y. Lee, Sheri Miraglia, Xiongwei Yan, Elana Swartzman, Susan Cornell-Kennon, Julia Mellentin-Michelotti, Charles Bruseo, Dennis S. France
Publikováno v:
Journal of biomolecular screening. 8(1)
High-throughput screening (HTS) for potential anticancer agents requires a broad portfolio of assay platforms that may include kinase enzyme assays, protein-protein binding assays, and functional cell-based apoptosis assays. The authors have explored
Autor:
Julia Mellentin-Michelotti, Pau-Miau Yuan, William E. Werner, Sheri Miraglia, Lolita Evangelista, Elana Swartzman
Publikováno v:
Analytical biochemistry. 272(2)
We have developed a fluorescence-based mix and read method for the quantitative determination of receptor-ligand binding interactions. This method was used to determine IC(50) values for peptide ligands of two endogenous seven-transmembrane receptors
Autor:
Mark Shannon, Hans Stoop, Leendert H. J. Looijenga, Wolter Oosterhuis, Astrid Ferlinz, Katharina Bierman, Elana Swartzman, Ad J. M. Gillis
Publikováno v:
Cancer Research. 70:1163-1163
Human testicular germ cell tumors (TGCTs) are the most frequent solid cancer in Caucasian males between the ages of 15 and 45 years. In spite of the high success rate of current treatment strategies (surgery, irradiation and chemotherapy), TGCTs are
Autor:
Thompson, Christopher M.1, Bloom, Lee R.1, Ogiue-Ikeda, Mari1, Machida, Kazuya1 machida@uchc.edu
Publikováno v:
BMC Biotechnology. 2015, Vol. 15 Issue 1, p1-13. 13p.