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pro vyhledávání: '"Elaine B. Shapland"'
Autor:
Elaine B. Shapland, Sunil S. Chandran
Publikováno v:
Methods in Molecular Biology ISBN: 9781493963416
The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cfce6b13e447f84df6e63bdf6942e165
https://doi.org/10.1007/978-1-4939-6343-0_14
https://doi.org/10.1007/978-1-4939-6343-0_14
Publikováno v:
Journal of Economic Entomology. 104:367-374
Almond leaf scorch (ALS) disease is caused by the bacterium Xylella fastidiosa and transmitted by xylem-feeding insects. Reports of increased incidence of ALS-diseased trees in California prompted surveys in three almond [Prunus dulcis (Mill.) D. A.
Autor:
Joseph H. Connell, Elaine B. Shapland, Kent M. Daane, Christina Wistrom, Glenn Y. Yokota, Roger A. Duncan, Mario Viveros
Publikováno v:
Plant Disease. 90:905-909
Xylella fastidiosa is a xylem-limited bacterium that causes almond leaf scorch (ALS), Pierce's disease of grapevines, and other plant diseases. We surveyed ground vegetation in ALS-infected almond orchards in California's Central Valley for the prese
Autor:
Sunil S. Chandran, Kedar G. Patel, Stefan de Kok, Jack D. Newman, Zach Serber, Leslie H. Stanton, Darren Platt, Victor Holmes, Maxime Durot, Todd Slaby, Jed Dean, Elaine B. Shapland
Publikováno v:
ACS synthetic biology. 3(2)
Assembly of DNA parts into DNA constructs is a foundational technology in the emerging field of synthetic biology. An efficient DNA assembly method is particularly important for high-throughput, automated DNA assembly in biofabrication facilities and
Publikováno v:
Current opinion in microbiology. 11(6)
Recent studies have illuminated key features of the transcriptional and signal transduction networks governing the cell division cycle of Caulobacter crescentus. These mechanisms generate oscillations in the activity of CtrA, a key regulator of DNA r
Autor:
Nicole Klinkner, Todd Slaby, Sunil S. Chandran, Elaine B. Shapland, Daniel Hollis, Kedar G. Patel, Yandi Dharmadi, Jed Dean
Publikováno v:
Nucleic Acids Research
DNA ‘assembly’ from ‘building blocks’ remains a cornerstone in synthetic biology, whether it be for gene synthesis (∼1 kb), pathway engineering (∼10 kb) or synthetic genomes (>100 kb). Despite numerous advances in the techniques used for