Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation

Autor: Narendra Padhan, Junhong Yan, Annegret Boge, Elaine Scrivener, Helgi Birgisson, Agata Zieba, Mats Gullberg, Masood Kamali-Moghaddam, Lena Claesson-Welsh, Ulf Landegren

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)

Abstract Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine. Capillary isoelectric focusing together with antibody-based detec

Externí odkaz: https://doaj.org/article/5fd5a922af6646128a84ed14eb0a59b9

Performance validation of an improved Xenon-arc lamp-based CCD camera system for multispectral imaging in proteomics

Autor: Patrick Courtney, Brett A. Boghigian, Elaine Scrivener, Mary F. Lopez, Alla Bogdanova, Eva Golenko, Eric Denoyer, Wayne F. Patton, Peter Jackson, Alvydas Mikulskis

Publikováno v: PROTEOMICS. 5:4354-4366

Advances in gel-based nonradioactive protein expression and PTM detection using fluorophores has served as the impetus for developing analytical instrumentation with improved imaging capabilities. We describe a CCD camera-based imaging instrument, eq

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d4be41b3733fa05294eac71902874a0
https://doi.org/10.1002/pmic.200500062

Analysis of the dynamic Bacillus subtilis Ser/thr/tyr phosphoproteome implicated in a wide variety of cellular processes

Autor: Peter Jackson, Elaine Scrivener, Alain Levine, Lauriane Kuhn, Patrick Courtney, Joëlle Vinh, Simone J. Séror, Jérôme Garin, Cédric Absalon, Valérie Labas, Françoise Vannier

Publikováno v: Proteomics
Proteomics, Wiley-VCH Verlag, 2006, 6, pp.2157
Proteomics, Wiley-VCH Verlag, 2006, 6 (7), pp.2157-73. ⟨10.1002/pmic.200500352⟩
HAL
Proteomics, 2006, 6 (7), pp.2157-73. ⟨10.1002/pmic.200500352⟩

The physiological role of proteins phosphorylated on serine/threonine/tyrosine (Ser/Thr/Tyr) residues or the identity of the corresponding kinases and phosphatases is generally poorly understood in bacteria. As a first step in analysing the importanc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd3c1105b63329f846a0ce13724eae8f
https://hal.archives-ouvertes.fr/hal-00067850

Peptidomics: A new approach to affinity protein microarrays

Autor: Elaine Scrivener, George Masih, Richard Barry, Patrick Hextall, Robert Calvert, Jonathan Terrett, Mikhail Soloviev, Albert Edward Platt

Publikováno v: Proteomics. 3(2)

Protein microarrays for diagnostic and proteomic analyses are being developed using a number of different techniques for each of the steps required including immobilisation methods, assay and detection systems. This is extremely different to the deve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2e6804ecbb792d24715cdb23cc0bd5a
https://pubmed.ncbi.nlm.nih.gov/12601804

Matrix-assisted laser desorption/ionisation mass spectra of antibiotic and cryptand complexes of alkali metal ions: a comparison with electrospray ionisation mass spectra

Autor: Margaret M. Sheil, Stephen F. Ralph, Elaine Scrivener, Peter J. Derrick

Publikováno v: European Journal of Mass Spectrometry. 3:229

Matrix-assisted laser desorption/ionisation (MALDI) mass spectra of samples containing valinomycin and all of the alkali metal ions suggest that the relative concentrations of metal complexes in solution play a role in determining relative peak inten

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5592ff23c1a9753530fc6467bd342aa5
https://doi.org/10.1255/ejms.16