Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Ekaterina Protozanova"'
Publikováno v:
Lab Chip. 13:240-251
Genome Sequence Scanning (GSS) is a bacterial identification technology that detects sparse sequence-specific fluorescent tags on long DNA molecules linearized in a continuous-flow microfunnel. The efficiency and sensitivity of GSS depends on the det
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df5ccf0cea1a0cc7f2ae330bcbc6e342
https://doi.org/10.1039/c2lc40943g
https://doi.org/10.1039/c2lc40943g
Autor:
Eric J. White, Sergey V. Fridrikh, Ekaterina Protozanova, Dirk Peter Ten Broeck, Douglas B. Cameron, Rudolf Gilmanshin, Emilia Mollova, Meng Zhang
Publikováno v:
Analytical Biochemistry. 402:83-90
Here we describe bacterial genotyping by direct linear analysis (DLA) single-molecule mapping. DLA involves preparation of restriction digest of genomic DNA labeled with a sequence-specific fluorescent probe and stained nonspecifically with intercala
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41402c6687ce1bbf16dd4cfdc19933eb
https://doi.org/10.1016/j.ab.2010.03.024
https://doi.org/10.1016/j.ab.2010.03.024
Publikováno v:
Analytical Biochemistry. 391:135-143
Existing methods for extraction and processing of large fragments of bacterial genomic DNA are manual, time-consuming, and prone to variability in DNA quality and recovery. To solve these problems, we have designed and built an automated fluidic syst
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b3b0f2691b2b56129db7c7069f865c8
https://doi.org/10.1016/j.ab.2009.05.008
https://doi.org/10.1016/j.ab.2009.05.008
Publikováno v:
Biophysical Journal. 90(9):3091-3099
Preservation of genetic information in DNA relies on shielding the nucleobases from damage within the double helix. Thermal fluctuations lead to infrequent events of the Watson-Crick basepair opening, or DNA “breathing”, thus making normally buri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::450137c983c4078d9731f326feadd8ed
Autor:
Maxim D. Frank-Kamenetskii, Ekaterina Protozanova, Viatcheslav A. Soldatenkov, Sergey Chasovskikh, Vadim V. Demidov
Publikováno v:
EMBO reports. 3:956-961
DNA looping is one of the key factors allowing proteins bound to different DNA sites to signal one another via direct contacts. We demonstrate that DNA looping can be generated in an arbitrary chosen site by sequence-directed targeting of double-stra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1519a58897795fa357e6d6c35221034
https://doi.org/10.1093/embo-reports/kvf192
https://doi.org/10.1093/embo-reports/kvf192
Kinetics and mechanism of the DNA double helix invasion by pseudocomplementary peptide nucleic acids
Autor:
Christopher Price, Ekaterina Protozanova, Konstantin I. Izvolsky, Maxim D. Frank-Kamenetskii, Peter E. Nielsen, Vadim V. Demidov
Publikováno v:
Proceedings of the National Academy of Sciences. 99:5953-5958
If adenines and thymines in two mutually complementary mixed-base peptide nucleic acid (PNA) oligomers are substituted with diaminopurines and thiouracils, respectively, so-called pseudocomplementary PNAs (pcPNAs) are created. Pairs of pcPNAs have re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0105589a2960c72d0d3d50c4f1dad042
https://doi.org/10.1073/pnas.092127999
https://doi.org/10.1073/pnas.092127999
Publikováno v:
Nano Letters. 2:269-274
New types of self-assembled 2-D and 3-D DNA networks are presented and characterized using scanning force microscopy. The building blocks of these networks are guanine-rich DNA sequences, d(A15G15) and biotinylated-d(A15G15). These sequences self-ass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::06877571bcf971e8505c4a283ad1a611
https://doi.org/10.1021/nl015672h
https://doi.org/10.1021/nl015672h
Autor:
Sean J. Gerrin, Gene Malkin, Griffis Joshua, Robert H. Meltzer, Ekaterina Protozanova, Shilpi P. Vyas, Mikhail M. Safranovitch
Publikováno v:
Lab on a chip. 14(19)
Sequence-nonspecific staining of DNA with intercalating fluorophores is required for fluorescence-based length estimation of elongated DNA in optical mapping techniques. However, the observed length of a DNA molecule is affected by the relative conce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2f053337a4a1e706d8f5cced6d6a0c7
https://pubmed.ncbi.nlm.nih.gov/25133764
https://pubmed.ncbi.nlm.nih.gov/25133764
Publikováno v:
Biopolymers. 58:355-358
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::30d56814f53afc578170b69733676c09
https://doi.org/10.1002/1097-0282(20010405)58:4<355::aid-bip1012>3.0.co
https://doi.org/10.1002/1097-0282(20010405)58:4<355::aid-bip1012>3.0.co
Publikováno v:
Biophysical Chemistry. 84:137-147
Dynamic light scattering has been used to study the formation of stable multistranded DNA complexes called frayed wires. DNA frayed wires arise from the indefinite self-association of oligonucleotides with long terminal tracks of guanines, e.g. d(A15
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cb5ff71ac1621ace6c239518acd11f2
https://doi.org/10.1016/s0301-4622(00)00105-8
https://doi.org/10.1016/s0301-4622(00)00105-8