Zobrazeno 1 - 10
of 115
pro vyhledávání: '"Eishiro Shikata"'
Autor:
Yoko Kawaguchi-Ito, Shi-Fang Li, Masaya Tagawa, Hiroyuki Araki, Masafumi Goshono, Shingen Yamamoto, Mayumi Tanaka, Masako Narita, Kazuaki Tanaka, Sheng-Xue Liu, Eishiro Shikata, Teruo Sano
Publikováno v:
PLoS ONE, Vol 4, Iss 12, p e8386 (2009)
Hop stunt was a mysterious disorder that first emerged in the 1940s in commercial hops in Japan. To investigate the origin of this disorder, we infected hops with natural Hop stunt viroid (HpSVd) isolates derived from four host species (hop, grapevin
Externí odkaz:
https://doaj.org/article/4ff4716b5bf344afac4a8151c5062e42
Autor:
Chikako Hiraishi, Kazusato Ohshima, Kazuya Sako, Kazutoshi Matsuo, Tetsuji Ogawa, Akio Nakagawa, Eishiro Shikata, Nobumichi Sako
Publikováno v:
Plant Disease. 84:1109-1115
Unfamiliar necrotic symptoms on or within potato tubers of cultivars Nishiyutaka and Dejima were observed in Nagasaki prefecture, Japan, in 1992. Symptoms were typically on the surface of the tuber, which either protruded at first and then became sun
Publikováno v:
Archives Of Phytopathology And Plant Protection. 32:179-192
The sensitivity of gene diagnostic methods for detecting chrysanthemum stunt viroid (CSVd) in 2 M LiCl‐soluble nucleic acids extracted from chrysanthemum plants was examined. Dot blot hybridization using DIG‐labeled cRNA and cDNA probes was compa
Autor:
Eishiro Shikata, Hayashi Yoshinori, Kenji S. Nakahara, Tatsuji Hataya, Takahiro Sugimoto, Ikuo Kimura
Publikováno v:
Journal of Virological Methods. 71:219-227
Five kinds of synthetic oligonucleotide probes labeled with biotin (BIO) were designed for the detection of potato spindle tuber viroid (PSTVd), and their sensitivities were compared with that of a digoxigenin (DIG)- or BIO-labeled cDNA probe. Althou
Autor:
Sachio Ohnuma, Teruo Sano, Tadashi Ogata, Shi-Fang Li, Chiyokichi Suzuki, Masafumi Ochiai, Eishiro Shikata
Publikováno v:
Japanese Journal of Phytopathology. 63:89-94
A viroid was detected from European pears cultivated in Fukushima and Yamagata prefectures, Japan. Nucleotide sequence analysis of each of the Fukushima and Yamagata isolates revealed that they were isolates of pear blister canker viroid (PBCVd) repo
Autor:
Ikuo Kimura, Ichiro Uyeda, Tatsuji Hataya, Eishiro Shikata, Haruhisa Suga, Sang Yong Lee, Jin Yan
Publikováno v:
Journal of General Virology. 76:975-978
The complete nucleotide sequence of rice ragged stunt virus genome segment 9 (S9) was determined. The S9 segment is 1132 nucleotides long and has a long open reading frame starting from the first AUG codon at nucleotide position 14-16 and terminating
Publikováno v:
Japanese Journal of Phytopathology. 61:381-390
Return-PAGE was reliable for diagnosis of HSVd in hop, CEVd in citron and ASSVd in apple, but was not reliable for HSVd in grapevine and citron, and ASSVd in pear, because of their low concentration. A disadvantage of return-PAGE was possible appeara
Publikováno v:
Japanese Journal of Phytopathology. 61:513-518
By polyacrylamide gel electrophoresis (PAGE) and nucleic acid hybridization, the extent of genomic relatedness between rice black-streaked dwarf Fijivirus (RBSDV) and maize rough dwarf Fijivirus (MRDV) was investigated. It was previously reported tha
Molecular Cloning and Complete Nucleotide Sequence of Rice Ragged Stunt Oryzavirus Genome Segment 10
Publikováno v:
Japanese Journal of Phytopathology. 61:189-193
cDNAs to rice ragged stunt Oryzavirus genome segment 10 (S10) were made by oligo-dT primed 1st strand cDNA synthesis after poly A addition to 3' terminal of viral transcripts and cloned in a bacterial plasmid pBR322. The overlapping cDNA clones were
Autor:
Takaaki Nakaya, Eishiro Shikata, Akihiro Matsuura, Kokichi Kikuchi, Kazusato Ohshima, Ikuo Kimura
Publikováno v:
Japanese Journal of Phytopathology. 60:600-607
cDNA to a plant virus-monoclonal antibody gene was cloned and sequenced. The source of mRNAs was a hybridoma producing an IgG3 monoclonal antibody (42C07) which bound to a coat protein of potato virus Y. We have constructed cDNA libraries using a bac