Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Einar Everitt"'
Autor:
Einar Everitt, Helena Fridholm
Publikováno v:
Journal of Fish Diseases. 34:663-675
The possible importance of the O-linked glycosylation in virion stability and infectivity of infectious pancreatic necrosis virus (IPNV) was analysed. Enzymatic treatment with O-glycosidase of radiolabelled virions under different ionic conditions, t
Publikováno v:
Viral Immunology. 20:635-648
The sole coat protein VP2 of infectious pancreatic necrosis virus (IPNV) was isolated and purified from intact virions, propagated in CHSE-214 cells. Likewise was the full-length VP2 protein isolated and purified upon cloning and expression of the co
Autor:
Einar Everitt, Helena Fridholm
Publikováno v:
Journal of Virological Methods. 128:67-71
The standard method for measuring the number of infectious phages in solution has traditionally been the plaque forming assay. An alternative method is described where the number of lytic, infectious phages is determined in an endpoint titration assa
Publikováno v:
Journal of Virological Methods. 110:173-178
A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 l water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger.
Publikováno v:
Virology. 274(2):367-377
Avian reovirus protein sigmaC, the viral cell-attachment protein, is a minor component of the outer-capsid shell of the viral particle that is synthesized in small amounts in infected cells. We cloned the sigmaC-encoding ORF in vector pIL-2f, express
Autor:
A. Hjalmarsson, Einar Everitt
Publikováno v:
Archives of Virology. 144:1487-1501
Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low a
Publikováno v:
Journal of Virological Methods. 80:17-24
Some cell lines are difficult to grow in confluent monolayers and, therefore, the plaque assay cannot be applied since plaques may be hard to distinguish from other blank areas of the cell monolayers. To avoid this problem a rapid and sensitive immun
Autor:
Joachim Regnéll, Einar Everitt
Publikováno v:
Vegetation History and Archaeobotany. 5:201-205
Methods of preparative centrifugation eliminate many of the difficulties involved in preparing pollen concentrates from deposits rich in resistant organic material. Density centrifugation for the separation of pollen from a gyttja sample rich in resi
Autor:
Torsten Gunnarsson, Hans-Börje Jansson, Eric Carlemalm, Einar Everitt, Göran Odham, Helena Åkesson
Publikováno v:
Fungal Genetics and Biology. 20:205-216
Akesson, H., Carlemalm, E., Everitt, E., Gunnarsson, T., Odham, G., and Jansson, H.-B. 1996. Immunocytochemical localization of phytotoxins inBipolaris sorokiniana. Fungal Genetics and Biology20,205–216. Polyclonal antibodies against prehelminthosp
Publikováno v:
Journal of Virological Methods. 47:27-35
An improved method for the isolation and purification of infectious pancreatic necrosis virus (IPNV) is described. Virions released into the clarified growth medium are adsorbed to an anion exchange resin of diethylaminoethyl cellulose at pH 8.1. IPN