Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Eiko Kanaya"'
Autor:
Hiroyuki Okano, Masashi Ozaki, Eiko Kanaya, Joong-Jae Kim, Clement Angkawidjaja, Yuichi Koga, Shigenori Kanaya
Publikováno v:
FEBS Open Bio, Vol 4, Iss C, Pp 936-946 (2014)
Ten genes encoding novel cellulases with putative signal peptides at the N-terminus, termed pre-LC-CelA–J, were isolated from a fosmid library of a leaf–branch compost metagenome by functional screening using agar plates containing carboxymethyl
Externí odkaz:
https://doaj.org/article/5b46f98a8e864669b2da311e581e3f49
Publikováno v:
Protein Science. 24:408-419
A metagenome-derived glycoside hydrolase family 9 enzyme with an N-terminal immunoglobulin-like (Ig-like) domain, leaf-branch compost (LC)-CelG, was characterized and its crystal structure was determined. LC-CelG did not hydrolyze p-nitrophenyl cello
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::37f04df9c7f28f4db0ecb18c6da8d731
https://doi.org/10.1002/pro.2632
https://doi.org/10.1002/pro.2632
Publikováno v:
Protein Science. 24:93-104
The genes encoding six novel esterolytic/lipolytic enzymes, termed LC-Est1∼6, were isolated from a fosmid library of a leaf-branch compost metagenome by functional screening using tributyrin agar plates. These enzymes greatly vary in size and amino
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7948d9d869a8ebd0941fda6bc9bd8aa6
https://doi.org/10.1002/pro.2591
https://doi.org/10.1002/pro.2591
Autor:
J. H. Kim, Clement Angkawidjaja, Hiroyuki Okano, Shigenori Kanaya, Eiko Kanaya, Masashi Ozaki, Yuichi Koga
Publikováno v:
FEBS Open Bio
FEBS Open Bio, Vol 4, Iss C, Pp 936-946 (2014)
FEBS Open Bio, Vol 4, Iss C, Pp 936-946 (2014)
Highlights • Ten novel cellulases, LC-CelA–J, were isolated from leaf–branch compost by a metagenomic approach. • LC-CelA was characterized. • The structure, activity, and stability of LC-CelA were similar to those of Cel12A from Rhodotherm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c18814e4da95b4506b21a85f37edcc3
http://europepmc.org/articles/PMC4239480
http://europepmc.org/articles/PMC4239480
Publikováno v:
FEBS Letters. 587:1418-1423
The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59440077c25c524bbea1c1019c17b93e
https://doi.org/10.1016/j.febslet.2013.03.020
https://doi.org/10.1016/j.febslet.2013.03.020
Autor:
Shigenori Kanaya, Nujarin Jongruja, Dong-Ju You, Eiko Kanaya, Clement Angkawidjaja, Yuichi Koga
Publikováno v:
FEBS Journal. 279:2737-2753
The crystal structure of ribonuclease H3 from Aquifex aeolicus (Aae-RNase H3) was determined at 2.0 A resolution. Aae-RNase H3 consists of an N-terminal TATA box-binding protein (TBP)-like domain (N-domain) and a C-terminal RNase H domain (C-domain).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7827c0c387bd5fc2fda115d6267b11d8
https://doi.org/10.1111/j.1742-4658.2012.08657.x
https://doi.org/10.1111/j.1742-4658.2012.08657.x
Autor:
Eiko Kanaya, Shigenori Kanaya, Kazufumi Takano, Tri-Nhan Nguyen, Clement Angkawidjaja, Yuichi Koga
Publikováno v:
Protein Science. 21:553-561
Metagenome-derived LC11-RNase H1 is a homolog of Sulfolobus tokodaii RNase H1 (Sto-RNase H1). It lacks a C-terminal tail, which is responsible for hyperstabilization of Sto-RNase H1. Sto-RNase H1 is characterized by its ability to cleave not only an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45b5665f3d36b487586ee6b5c92b2fbb
https://doi.org/10.1002/pro.2043
https://doi.org/10.1002/pro.2043
Autor:
J. H. Kim, Shigenori Kanaya, Eiko Kanaya, Kazufumi Takano, Che Ok Jeon, Hyo Jung Lee, Yuichi Koga, Hyun Mi Jin
Publikováno v:
International Journal of Systematic and Evolutionary Microbiology. 61:1595-1600
A strictly aerobic, Gram-stain-negative, yellow-pigmented, non-spore-forming, motile (by gliding), rod-shaped bacterium, designated strain 15F3T, was isolated from leaf-and-branch compost. Phylogenetic analysis based on 16S rRNA gene sequences showed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c39314012a96fc84eafaf31878492798
https://doi.org/10.1099/ijs.0.022467-0
https://doi.org/10.1099/ijs.0.022467-0
Publikováno v:
FEBS Journal. 277:4474-4489
Thermotoga maritima ribonuclease H (RNase H) I (Tma-RNase HI) contains a hybrid binding domain (HBD) at the N-terminal region. To analyze the role of this HBD, Tma-RNase HI, Tma-W22A with the single mutation at the HBD, the C-terminal RNase H domain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::84550ca6915d3d1ced9aac621d351715
https://doi.org/10.1111/j.1742-4658.2010.07834.x
https://doi.org/10.1111/j.1742-4658.2010.07834.x
Autor:
N.T. Nguyen, Eiko Kanaya, Shigenori Kanaya, S. Koikeda, Yuichi Koga, Kazufumi Takano, T. Sakabe
Publikováno v:
Journal of Applied Microbiology. 109:974-983
Aims: The study aimed to combine a metagenomics approach with complementary genetics to identify novel bacterial genes with orthologous functions, with the identification of novel RNase H genes as a test case. Methods and Results: A metagenomic DNA l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07b011de71d0422b859541ba0643844e
https://doi.org/10.1111/j.1365-2672.2010.04724.x
https://doi.org/10.1111/j.1365-2672.2010.04724.x