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pro vyhledávání: '"Eiju Sasaki"'
Publikováno v:
Journal of bioscience and bioengineering. 111(5)
In the present study, we investigated a microplate-based culture (MBC) of Escherichia coli for the high-throughput, high-level production of PS-tag-fused scFvs (scFv-PS) in insoluble form. The Overnight Express™ Autoinduction System (OE system) was
Autor:
Yuki Shiritani, Eiju Sasaki, Aya Nakagawa, Kyoko Hamasaki, Michimasa Kishimoto, Yoichi Kumada
Publikováno v:
Analytical and bioanalytical chemistry. 398(3)
In the present study, we demonstrated site-specific immobilization and solid-phase refolding of single-chain Fv antibodies on hydrophilic polystyrene (phi-PS) plates that was mediated by novel polystyrene binding peptides (PS-tags: RIIIRRIRR), which