Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Ehud Sass"'
Autor:
Benjamin C. Buchmuller, Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Ehud Sass, Emmanuel D. Levy, Michael Knop
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Construction of yeast libraries is time-consuming, costly and limited to the genetic background of the chosen strain. Here the authors present CASTLING which uses CRISPR-Cas12a and oligonucleotide pools to rapidly generate pooled libraries with large
Externí odkaz:
https://doaj.org/article/a27b637012fa456695e303da14f899ec
Autor:
Sivan Kaminski Strauss, Dvir Schirman, Ghil Jona, Aaron N Brooks, Aditya M Kunjapur, Alex N Nguyen Ba, Alice Flint, Andras Solt, Andreas Mershin, Atray Dixit, Avihu H Yona, Bálint Csörgő, Bede Phillip Busby, Bianca P Hennig, Csaba Pál, Daniel Schraivogel, Daniel Schultz, David G Wernick, Deepa Agashe, Dikla Levi, Dmitry Zabezhinsky, Dor Russ, Ehud Sass, Einat Tamar, Elad Herz, Emmanuel D Levy, George M Church, Idan Yelin, Iftach Nachman, Jeffrey E Gerst, Joseph M Georgeson, Katarzyna P Adamala, Lars M Steinmetz, Marc Rübsam, Markus Ralser, Michael Klutstein, Michael M Desai, Nilima Walunjkar, Ning Yin, Noa Aharon Hefetz, Noah Jakimo, Olga Snitser, Omri Adini, Prashant Kumar, Rachel Soo Hoo Smith, Razi Zeidan, Ronen Hazan, Roni Rak, Roy Kishony, Shannon Johnson, Shira Nouriel, Sibylle C Vonesch, Simmie Foster, Tal Dagan, Tanita Wein, Thrasyvoulos Karydis, Timothy M Wannier, Timothy Stiles, Viridiana Olin-Sandoval, William F Mueller, Yinon M Bar-On, Orna Dahan, Yitzhak Pilpel
Publikováno v:
PLoS Biology, Vol 17, Iss 3, p e3000182 (2019)
In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporadically? Sho
Externí odkaz:
https://doaj.org/article/bb9a50be8dcc4dc98274ddfdc59bba88
Autor:
Matthias Meurer, Benjamin C. Buchmuller, Ehud Sass, Konrad Herbst, Emmanuel D. Levy, Michael Knop, Daniel Kirrmaier
Publikováno v:
Nature Communications
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Clone collections of modified strains (“libraries”) are a major resource for systematic studies with the yeast Saccharomyces cerevisiae. Construction of such libraries is time-consuming, costly and confined to the genetic background of a specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3378a682cf71ccd5318ac6735f7c707a
http://europepmc.org/articles/PMC6609715
http://europepmc.org/articles/PMC6609715
Autor:
David G. Wernick, Michael Klutstein, Roni Rak, Shannon M. Johnson, Andreas Mershin, Daniel Schultz, Simmie L. Foster, Sibylle C. Vonesch, Yinon M. Bar-On, Daniel Schraivogel, Jeffrey E. Gerst, Olga Snitser, Yitzhak Pilpel, Katarzyna P. Adamala, Dmitry Zabezhinsky, Idan Yelin, Atray Dixit, Markus Ralser, Bianca P. Hennig, Orna Dahan, Dvir Schirman, Dikla Levi, Aditya M. Kunjapur, Roy Kishony, Sivan Kaminski Strauss, Bede P. Busby, Joseph M. Georgeson, Omri Adini, Ghil Jona, Alex N. Nguyen Ba, Ronen Hazan, Shira Nouriel, Lars M. Steinmetz, Timothy Stiles, Tal Dagan, Timothy M. Wannier, William F. Mueller, Thrasyvoulos Karydis, Csaba Pál, Ehud Sass, Noah Jakimo, Marc Rübsam, George M. Church, Alice Flint, Tanita Wein, Prashant Kumar, Nilima Walunjkar, Viridiana Olin-Sandoval, Aaron N. Brooks, Dor Russ, Andras Solt, Iftach Nachman, Einat Shaer Tamar, Michael M. Desai, Bálint Csörgő, Noa Aharon Hefetz, Rachel Soo Hoo Smith, Deepa Agashe, Razi Zeidan, Ning Yin, Emmanuel D. Levy, Elad Herz, Avihu H. Yona
Publikováno v:
PLoS Biology
PLoS Biology, Vol 17, Iss 3, p e3000182 (2019)
PLoS Biology, Vol 17, Iss 3, p e3000182 (2019)
In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporadically? Sho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32c2152f7a9827795b86493d0005a3c5
Isogenic cells living in the same environment show a natural heterogeneity associated with fluctuations in gene expression. When these fluctuations propagate through cellular regulatory networks, they can give rise to noise regulons, whereby multiple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52769de642cdc88f51b6a5f1fe10ff16
https://doi.org/10.1101/260695
https://doi.org/10.1101/260695
Autor:
Yotam Nadav, Maya Schuldiner, Michael Knop, Ehud Sass, Emmanuel D. Levy, Uri Weill, Joseph M. Georgeson, Matthias Meurer, Yuanqiang Duan, Meta Heidenreich, Benjamin Dubreuil
Publikováno v:
Nucleic Acids Research
The ability to measure the abundance and visualize the localization of proteins across the yeast proteome has stimulated hypotheses on gene function and fueled discoveries. While the classic C’ tagged GFP yeast library has been the only resource fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55aed60ee15e19e4a4ddab1ab48a25b7
Autor:
Ilia Kats, Martin Štefl, Ehud Sass, Anton Khmelinskii, Verena Dederer, Konrad Herbst, Koen Van Laer, Emmanuel D. Levy, Florian Huber, Tobias P. Dick, Marius K. Lemberg, Benjamin C. Buchmuller, Yuanqiang Duan, Michael Knop, Daniel Kirrmaier, Matthias Meurer
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae ORFs. It consists of 5661 strains with an acceptor module inserted after each ORF, which can be efficiently replaced with tags or regulatory elements. We valida
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::813343810a9ffad87673864af80afe73
https://doi.org/10.1101/226811
https://doi.org/10.1101/226811
Autor:
Ehud Sass, Tobias P. Dick, Konrad Herbst, Michael Knop, Emmanuel D. Levy, Florian Huber, Yuanqiang Duan, Marius K. Lemberg, Ilia Kats, Anton Khmelinskii, Matthias Meurer, Verena Dederer, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Benjamin C. Buchmuller
Publikováno v:
Nature methods. 15(8)
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. W
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2816a641e8f52fabbaaae0aebcea3393
https://pubmed.ncbi.nlm.nih.gov/29988096
https://pubmed.ncbi.nlm.nih.gov/29988096
Autor:
Ophry Pines, Jacqueline Kowarzyk, Stephen W. Michnick, Ido Yofe, Shifra Ben-Dor, Zohar Avihou, Silvia G. Chuartzman, Reut Ben-Menachem, Einat Zalckvar, Bram Stynen, Maya Schuldiner, Doron Rapaport, Janina Laborenz, Nofar Harpaz, Barbara Knoblach, Omer Goldman, Janani Natarajan, Emmanuel D. Levy, Richard A. Rachubinski, Felix Boos, Dan Davidi, Ehud Sass, Johannes M. Herrmann, Uri Weill, Kiril Kniazev
Publikováno v:
Nature Methods. 16:205-205
The version of Supplementary Table 1 originally published online with this article contained incorrect localization annotations for one plate. This error has been corrected in the online Supplementary Information.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4f84b68994bc08815b83f99acfb22d6e
https://doi.org/10.1038/s41592-018-0297-3
https://doi.org/10.1038/s41592-018-0297-3
Publikováno v:
Nature Communications. 5
Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c68d507259e369ec737c78b0776e93e0
https://doi.org/10.1038/ncomms4574
https://doi.org/10.1038/ncomms4574