Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Egon Amann"'
Autor:
Ivan M. Blasutig, Sarah E. Wheeler, Renze Bais, Pradeep Kumar Dabla, Ji Lin, Armand Perret-Liaudet, Annette Thomas, Kandace A. Cendejas, Jean-Marc Giannoli, Anne Vassault, Egon Amann, Qing H. Meng
Publikováno v:
Clinical Chemistry and Laboratory Medicine (CCLM).
Objectives Clinical laboratory results are required for critical medical decisions, underscoring the importance of quality results. As part of total quality management, external quality assessment (EQA) is a vital component to ensure laboratory accur
The fast-growing Gram-negative bacteriumVibrio natriegensis an attractive host for a range of applications in molecular biology and biotechnology. Moreover, the remarkable speed of growth ofVibrio natriegensposes fundamental questions on bacterial ph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86831a1babac01920f30756693aebec7
Publikováno v:
Protein Expression and Purification. 6:305-311
Osteoblast-specific factor 2 (OSF-2) is a approximately 90-kDa protein selectively expressed in bone. OSF-2 cDNA was recently isolated from mouse and human cDNA libraries and shows limited sequence homology with fasciclin I, a cell adhesion protein e
Autor:
Shinji Kawai, Sunao Takeshita, Reiko Kikuno, Egon Amann, Atsushi Tsujimura, Akira Kudo, Makoto Okazaki
Publikováno v:
Journal of Biological Chemistry. 269:12092-12098
The preferential screening of cDNA libraries derived from the mouse osteoblastic cell line MC3T3-E1 has yielded a cDNA clone encoding 796 amino acids including the typical 24-residue-long signal sequence. The predicted amino acid sequence revealed th
Publikováno v:
Molecular Immunology. 30:1491-1498
Five human nuclear antigens, RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D, were produced in E. coli using the expression vector pSEM. cDNAs encoding these antigens were ligated to a truncated lacZ' gene of the vector and the beta-galactosidase fusion protein
Publikováno v:
Molecular Reproduction and Development. 34:349-356
It is a well-known phenomenon that cultured mammalian cells that have been pricked in the presence of foreign DNA can be transformed. This micromanipulation ‘pricking’ technique was applied to mouse blastocysts to determine whether uptake of exog
Publikováno v:
DNA and Cell Biology. 11:735-743
The mouse OSF-1 protein (also known as pleiotrophin, HB-GAM, HBGF-8, or HBNF) gene was isolated from a mouse genomic library and sequenced. OSF-1 is a 15-kD secreted protein specifically expressed in bone and brain, and is believed to play a role in
Autor:
Egon Amann, Birgit Lehmeier
Publikováno v:
Journal of Biotechnology. 23:153-165
Two new plasmid expression vectors, pTacT7 and pTacT7L, have been constructed, which incorporate between the tac promoter and a downstream NcoI-HindIII polylinker sequence a synthetic sequence derived from the region upstream from gene 10 of bacterio
Autor:
Takeyuki Sugiura, Egon Amann
Publikováno v:
Biotechnology and bioengineering. 51(4)
The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum-free culture conditions. Sf9 cells in SF900II medium apparently utilized sucrose as a primary nutrient both before and after virus infection, yielding small amounts of
Publikováno v:
Gene. 152(2)
The cDNAs encoding the porcine 65- and 67-kDa glutamic acid decar☐ylases (GAD 65 and GAD 67 , respectively) were cloned by the PCR method. The 2246-nucleotide (nt) GAD 65 cDNA contained an open reading frame (ORF) coding for a protein of 585 amino