Zobrazeno 1 - 10
of 1 188
pro vyhledávání: '"Ege T"'
Autor:
Natalie J. Guzikowski, Ege T. Kavalali
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract The mechanisms that enable synapses to achieve temporally and spatially precise signaling at nano-scale while being fluid with the cytosol are poorly understood. Liquid-liquid phase separation (LLPS) is emerging as a key principle governing
Externí odkaz:
https://doaj.org/article/71ad134f8f7b44799e024458d4c6de47
Autor:
Elena D. Bagatelas, Ege T. Kavalali
Publikováno v:
iScience, Vol 27, Iss 7, Pp 110176- (2024)
Summary: Homeostatic plasticity mechanisms act in a negative feedback manner to stabilize neuronal firing around a set point. Classically, homeostatic synaptic plasticity is elicited via rather drastic manipulation of activity in a neuronal populatio
Externí odkaz:
https://doaj.org/article/afc90760631c4794a4eb0813e6676f72
Publikováno v:
STAR Protocols, Vol 5, Iss 2, Pp 103089- (2024)
Summary: Generating stable human embryonic stem cells (hESCs) with targeted genetic mutations allows for the interrogation of protein function in numerous cellular contexts while maintaining a relatively high degree of isogenicity. We describe a step
Externí odkaz:
https://doaj.org/article/5e5744a3da6149a998a4cbad5d59afe3
Publikováno v:
Cell Reports, Vol 42, Iss 10, Pp 113201- (2023)
Summary: Calcium (Ca2+) signaling is tightly regulated within a presynaptic bouton. Here, we visualize Ca2+ signals within hippocampal presynaptic boutons using GCaMP8s tagged to synaptobrevin, a synaptic vesicle protein. We identify evoked presynapt
Externí odkaz:
https://doaj.org/article/4a7659bb7ce04609a2c6329aaacfaed8
Autor:
Klaudia Jaczynska, Luis Esquivies, Richard A. Pfuetzner, Baris Alten, Kyle D. Brewer, Qiangjun Zhou, Ege T. Kavalali, Axel T. Brunger, Josep Rizo
Publikováno v:
FEBS Open Bio, Vol 13, Iss 1, Pp 26-50 (2023)
Characterizing interactions of Synaptotagmin‐1 with the SNARE complex is crucial to understand the mechanism of neurotransmitter release. X‐ray crystallography revealed how the Synaptotagmin‐1 C2B domain binds to the SNARE complex through a so
Externí odkaz:
https://doaj.org/article/4c5a346bf0504f2d810b8e365e02f59e
Autor:
Burak Uzay, Ege T. Kavalali
Publikováno v:
Frontiers in Synaptic Neuroscience, Vol 15 (2023)
Synaptic neurotransmitter release is an evolutionarily conserved process that mediates rapid information transfer between neurons as well as several peripheral tissues. Release of neurotransmitters are ensured by successive events such as synaptic ve
Externí odkaz:
https://doaj.org/article/77b312943bad49898a5d0805eedd9d34
Autor:
Natalie J. Guzikowski, Ege T. Kavalali
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102080- (2023)
Summary: Visualizing the nano-organization of the synapse is fundamental to elucidating the structure-function relationship of the nervous system. The advent of super-resolution microscopy provides a tool to assess and quantify the dynamic organizati
Externí odkaz:
https://doaj.org/article/4b81fab869c14bbbad193dcaf895ebe4
Publikováno v:
Cell Reports, Vol 42, Iss 2, Pp 112042- (2023)
Summary: Rapid release of neurotransmitters in synchrony with action potentials is considered a key hardwired property of synapses. Here, in glutamatergic synapses formed between induced human neurons, we show that action potential-dependent neurotra
Externí odkaz:
https://doaj.org/article/fea03f4d189f4dc2a9f038f65a80da0b
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101443- (2022)
Summary: Immunolabeling of surface AMPA receptors (AMPARs) can be used for in vivo or ex vivo examination of synaptic scaling, a type of homeostatic plasticity. Here, we present a protocol to analyze changes in synaptic weights using immunohistochemi
Externí odkaz:
https://doaj.org/article/b98d09d26e5740ab817f12569d90d013
Publikováno v:
eLife, Vol 11 (2022)
Synapses maintain both action potential-evoked and spontaneous neurotransmitter release; however, organization of these two forms of release within an individual synapse remains unclear. Here, we used photobleaching properties of iGluSnFR, a fluoresc
Externí odkaz:
https://doaj.org/article/ba5f7accb51f4f63b4ac60f611f5e381