MESSAR: Automated recommendation of metabolite substructures from tandem mass spectra.

Autor: Youzhong Liu, Aida Mrzic, Pieter Meysman, Thomas De Vijlder, Edwin P Romijn, Dirk Valkenborg, Wout Bittremieux, Kris Laukens

Publikováno v: PLoS ONE, Vol 15, Iss 1, p e0226770 (2020)

Despite the increasing importance of non-targeted metabolomics to answer various life science questions, extracting biochemically relevant information from metabolomics spectral data is still an incompletely solved problem. Most computational tools t

Externí odkaz: https://doaj.org/article/910f46af140d426c94eaecd6008330bb

A tutorial in small molecule identification via electrospray ionization-mass spectrometry: The practical art of structural elucidation

Autor: Thomas De Vijlder, Filip Cuyckens, Kris Laukens, Filip Lemière, Edwin P. Romijn, Dirk Valkenborg

Publikováno v: Mass Spectrometry Reviews
Mass spectrometry reviews

The identification of unknown molecules has been one of the cornerstone applications of mass spectrometry for decades. This tutorial reviews the basics of the interpretation of electrospray ionization-based MS and MS/MS spectra in order to identify s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0844acbbe2c84bb52d007c24a236bdd6
https://doi.org/10.1002/mas.21551

Heterogeneity assessment of antibody-derived therapeutics at the intact and middle-up level by low-flow sheathless capillary electrophoresis-mass spectrometry

Autor: Govert W. Somsen, Rob Haselberg, Edwin P. Romijn, Thomas De Vijlder, Raimond Heukers, Elena Domínguez-Vega, Martine J. Smit

Publikováno v: Analytica Chimica Acta, 1044, 181-190. Elsevier
Haselberg, R, De Vijlder, T, Heukers, R, Smit, M J, Romijn, E P, Somsen, G W & Domínguez-Vega, E 2018, ' Heterogeneity assessment of antibody-derived therapeutics at the intact and middle-up level by low-flow sheathless capillary electrophoresis-mass spectrometry ', Analytica Chimica Acta, vol. 1044, pp. 181-190 . https://doi.org/10.1016/j.aca.2018.08.024

Antibody-based pharmaceuticals often encompass a complex structural heterogeneity requiring enhanced analytical methods for reliable characterization of variants and degradation products. We have explored the capabilities of low-flow sheathless capil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc45de44e59bbcac39f447883b70fccb
https://pubmed.ncbi.nlm.nih.gov/30442400

Molecular Architecture of the Centriole Proteome: The Conserved WD40 Domain Protein POC1 Is Required for Centriole Duplication and Length Control

Autor: John R. Yates, Wallace F. Marshall, Ivan Zamora, Stefan Geimer, Lani C. Keller, Edwin P. Romijn

Publikováno v: Molecular Biology of the Cell. 20:1150-1166

Centrioles are intriguing cylindrical organelles composed of triplet microtubules. Proteomic data suggest that a large number of proteins besides tubulin are necessary for the formation and maintenance of a centriole's complex structure. Expansion of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91c45f10a258c71a8d0b68d29667a298
https://doi.org/10.1091/mbc.e08-06-0619

Expression Clustering Reveals Detailed Co-expression Patterns of Functionally Related Proteins during B Cell Differentiation

Autor: Asier Fullaondo, Chantal Christis, Marnix Wieffer, Edwin P. Romijn, Albert J. R. Heck, Ineke Braakman, Peter van der Sluijs, Joost W. Gouw

Publikováno v: Molecular & Cellular Proteomics. 4:1297-1310

B cells play an essential role in the immune response. Upon activation they may differentiate into plasma cells that secrete specific antibodies against potentially pathogenic non-self antigens. To identify the cellular proteins that are important fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cf70f9fefdcefbba25045dcc31be6f22
https://doi.org/10.1074/mcp.m500123-mcp200