Zobrazeno 1 - 10
of 74
pro vyhledávání: '"Edward Moczydlowski"'
Autor:
Edward Moczydlowski
Publikováno v:
Toxicon. 63:165-183
In many respects tetrodotoxin (TTX) is the quintessential natural toxin. It is unequivocally toxic to mammals with LD(50) values for mice in the range of 10 μg/kg (intraperitoneal), 16 μg/kg (subcutaneous), and 332 μg/kg (oral) (Kao, 1966). Its bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7a9ca4f008f2e0e8165d58bd4dd4904
https://doi.org/10.1016/j.toxicon.2012.11.026
https://doi.org/10.1016/j.toxicon.2012.11.026
Publikováno v:
Channels. 6:41-49
Spermidine and spermine, are endogenous polyamines (PAs) that regulate cell growth and modulate the activity of numerous ion channel proteins. In particular, intracellular PAs are potent blockers of many different cation channels and are responsible
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad3c9c8840b4f99004236b4689ebc40b
https://doi.org/10.4161/chan.19001
https://doi.org/10.4161/chan.19001
Publikováno v:
The Journal of General Physiology
Journal of General Physiology, vol. 140, no. 4, pp. 435-454
Journal of General Physiology, vol. 140, no. 4, pp. 435-454
Voltage-gated Na+ channels (NaV channels) are specifically blocked by guanidinium toxins such as tetrodotoxin (TTX) and saxitoxin (STX) with nanomolar to micromolar affinity depending on key amino acid substitutions in the outer vestibule of the chan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17e3d98593285e9692190e62d62a6d59
http://europepmc.org/articles/PMC3457692
http://europepmc.org/articles/PMC3457692
Publikováno v:
Biochemistry. 47:5354-5367
The selectivity filter of most K+ channels contains a highly conserved Thr residue that uniquely forms the S4 binding site for K+ by dual coordination with the backbone carbonyl oxygen and side chain hydroxyl of the same residue. This study examines
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c698f30c36f968dc46ad391cfcb89897
https://doi.org/10.1021/bi702281p
https://doi.org/10.1021/bi702281p
Publikováno v:
Bioconjugate Chemistry. 17:689-699
Iberiotoxin (IbTx) is a scorpion venom peptide that inhibits BK Ca2+-activated K+ channels with high affinity and specificity. Automated solid-phase synthesis was used to prepare a biotin-labeled derivative (IbTx-LC-biotin) of IbTx by substitution of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::707c189b71542ad6362a3ee2d7f3ed9e
https://doi.org/10.1021/bc060002u
https://doi.org/10.1021/bc060002u
Autor:
John Down, Alun Jones, Bruce G. Livett, Natalie M. Broxton, Jon-Paul Bingham, Edward Moczydlowski
Publikováno v:
Analytical Biochemistry. 338:48-61
We describe a strategy for the efficient, unambiguous assignment of disulfide connectivities in alpha-conotoxin SII, of which approximately 30% of its mass is cysteine, as an example of a generalizable technique for investigation of cysteine-rich pep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e46742ec7679937871c92c6649bb6476
https://doi.org/10.1016/j.ab.2004.10.001
https://doi.org/10.1016/j.ab.2004.10.001
Publikováno v:
The Journal of General Physiology
Crystal structures of the tetrameric KcsA K+ channel reveal seven distinct binding sites for K+ ions within the central pore formed at the fourfold rotational symmetry axis. Coordination of an individual K+ ion by eight protein oxygen atoms within th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9363f8ad36dfeabf5c5c4875c7e018a7
http://europepmc.org/articles/PMC2266582
http://europepmc.org/articles/PMC2266582
Publikováno v:
Journal of General Physiology. 124:691-701
According to the classic modulated receptor hypothesis, local anesthetics (LAs) such as benzocaine and lidocaine bind preferentially to fast-inactivated Na+ channels with higher affinities. However, an alternative view suggests that activation of Na+
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d92fc470c591a86760dc79e9e77c2122
https://doi.org/10.1085/jgp.200409128
https://doi.org/10.1085/jgp.200409128
Publikováno v:
Receptors and Channels. 10:131-138
After transient transfection of an hNav1.4-L443C/A444W mutant clone, HEK-293 cells exhibited large inactivation-deficient Na+currents. We subsequently established a stable cell line expressing robust inactivation-deficient Na+currents. Persistent lat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45901d202d6feddd5f5fdebb7fa490c2
https://doi.org/10.3109/10606820490514914
https://doi.org/10.3109/10606820490514914
Publikováno v:
Journal of General Physiology. 121:375-398
Incorporation of BK Ca2+–activated K+ channels into planar bilayers composed of negatively charged phospholipids such as phosphatidylserine (PS) or phosphatidylinositol (PI) results in a large enhancement of unitary conductance (gch) in comparison
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::34c33f48c9485d872adeefd63c67cc23
https://doi.org/10.1085/jgp.200208746
https://doi.org/10.1085/jgp.200208746