Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Edward J. Mullaney"'
Publikováno v:
Annual Review of Animal Biosciences. 1:283-309
Phytases are phosphohydrolytic enzymes that initiate stepwise removal of phosphate from phytate. Simple-stomached species such as swine, poultry, and fish require extrinsic phytase to digest phytate, the major form of phosphorus in plant-based feeds.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dad4955e6917fff234f51c53e179a7c
https://doi.org/10.1146/annurev-animal-031412-103717
https://doi.org/10.1146/annurev-animal-031412-103717
Publikováno v:
Advances in Microbiology. :395-398
Despite yeast having its own native phytase, the high levels of phytate found in DDGS, a byproduct of ethanol (ETOH) fermentation, suggest that its activity is diminished in the presence of ETOH. Ethanol, a product of grain fermentation, is known to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fc77db93a00820812bdd4253cede0556
https://doi.org/10.4236/aim.2012.23050
https://doi.org/10.4236/aim.2012.23050
Publikováno v:
Journal of Agricultural and Food Chemistry. 56:8179-8183
The role of disulfide bridges in the folding of Aspergillus niger phytase pH 2.5-optimum (PhyB) was investigated using dynamic light scattering (DLS). Guanidinium chloride (GuCl) at 1.0 M unfolded phytase; however, its removal by dialysis refolded th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7554b49b4e67d559df2538a891d9a670
https://doi.org/10.1021/jf8013712
https://doi.org/10.1021/jf8013712
Publikováno v:
Applied and Environmental Microbiology. 73:3069-3076
Although it has been widely used as a feed supplement to reduce manure phosphorus pollution of swine and poultry, Aspergillus niger PhyA phytase is unable to withstand heat inactivation during feed pelleting. Crystal structure comparisons with its cl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89e70b5261441869e3f250c53aa43146
https://doi.org/10.1128/aem.02970-06
https://doi.org/10.1128/aem.02970-06
Autor:
Sandra Austin-Phillips, Thomas Ziegelhoffer, Abul H. J. Ullah, Edward J. Mullaney, Kandan Sethumadhavan
Publikováno v:
Biochemical and Biophysical Research Communications. 290:1343-1348
The phyA gene from Aspergillus ficuum that codes for a 441-amino-acid full-length phosphomonoesterase (phytase) was cloned and expressed in Medicago sativa (alfalfa) leaves. The expressed enzyme from alfalfa leaves was purified to homogeneity and bio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fcc956f9722d8353701aa8bb0ea50c6
https://doi.org/10.1006/bbrc.2002.6361
https://doi.org/10.1006/bbrc.2002.6361
Publikováno v:
Biochemical and Biophysical Research Communications. 268:373-378
Aspergillus fumigatus phytase is a heat-stable enzyme of great potential. Our objective was to determine if a high level of functional expression of the A. fumigatus phytase gene could be produced in Pichia pastoris and how the recombinant phytase re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d81c8a94b4975046cbe8a09a7056b19
https://doi.org/10.1006/bbrc.2000.2121
https://doi.org/10.1006/bbrc.2000.2121
Autor:
Edward J. Mullaney, Brian Q. Phillippy
Publikováno v:
Journal of Agricultural and Food Chemistry. 45:3337-3342
The gene (phyA) for the Aspergillus niger phytase with optima at pH 5.5 and 2.2 was expressed in Escherichia coli under the control of the T7lac promoter. A 56 kDa fusion protein comprised of phytase linked to an S-Tag leader peptide accumulated in i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2d4dd78872b6ce279ef86e48f36901e9
https://doi.org/10.1021/jf970276z
https://doi.org/10.1021/jf970276z
Autor:
Edward J. Mullaney, Abul H. J. Ullah
Publikováno v:
Biochemical and Biophysical Research Communications. 227:311-317
The function of disulfide bonds in Aspergillus ficuum phytase was elucidated by unfolding studies, using guanidinium hydrochloride (Gu.HCl) as denaturant. Although the enzyme is totally inactivated by 0.8 M Gu.HCl, at pH 5.0, the active conformation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35903b5bf7a7743761912121338e39b7
https://doi.org/10.1006/bbrc.1996.1506
https://doi.org/10.1006/bbrc.1996.1506
Autor:
Deepak Bhatnagar, Jiujiang Yu, Thomas E. Cleveland, Perng-Kuang Chang, M. A. Klich, Edward J. Mullaney
Publikováno v:
Applied Microbiology and Biotechnology. 44:439-443
Southern blots of DNA from a number of aspergilli belonging to Aspergillus section Flavi, including aflatoxin-producing and non-aflatoxigenic isolates of A. flavus and A. parasiticus, were probed with the aflatoxin pathway genes aflR and omt-1. DNA o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abd515e13148b925ca552a925842575c
https://doi.org/10.1007/bf00169941
https://doi.org/10.1007/bf00169941
Publikováno v:
Gene. 162:117-121
We have used the Aspergillus niger (An) aphA gene as a probe and cloned the A. ficuum (Af) SRRC 265 gene encoding an extracellular pH 6.0-optimum acid phosphatase (APase6) from a genomic library. The identity of the Af aphA gene was confirmed and its
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05d5fff60055ea8ad6b2634082e16401
https://doi.org/10.1016/0378-1119(95)00298-k
https://doi.org/10.1016/0378-1119(95)00298-k