Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics

Autor: Bert Dhondt, Cláudio Pinheiro, Edward Geeurickx, Joeri Tulkens, Glenn Vergauwen, Edwin Van Der Pol, Rienk Nieuwland, Anneleen Decock, Ilkka Miinalainen, Pekka Rappu, Gary Schroth, Scott Kuersten, Jo Vandesompele, Pieter Mestdagh, Nicolaas Lumen, Olivier De Wever, An Hendrix

Publikováno v: Journal of Extracellular Vesicles, Vol 12, Iss 5, Pp n/a-n/a (2023)

Abstract The analysis of extracellular vesicles (EV) in blood samples is under intense investigation and holds the potential to deliver clinically meaningful biomarkers for health and disease. Technical variation must be minimized to confidently asse

Externí odkaz: https://doaj.org/article/88cf6c37fc744c8b910af6ec08d4058f

FO‐SPR biosensor calibrated with recombinant extracellular vesicles enables specific and sensitive detection directly in complex matrices

Autor: Yagmur Yildizhan, Venkata Suresh Vajrala, Edward Geeurickx, Charles Declerck, Nevena Duskunovic, Delphine De Sutter, Sam Noppen, Filip Delport, Dominique Schols, Johannes V. Swinnen, Sven Eyckerman, An Hendrix, Jeroen Lammertyn, Dragana Spasic

Publikováno v: Journal of Extracellular Vesicles, Vol 10, Iss 4, Pp n/a-n/a (2021)

Abstract Extracellular vesicles (EVs) have drawn huge attention for diagnosing myriad of diseases, including cancer. However, the EV detection and analyses procedures often lack much desired sample standardization. To address this, we used well‐cha

Externí odkaz: https://doaj.org/article/4e8411d58d5f41989eb27ca1ef801402

Recombinant extracellular vesicles as biological reference material for method development, data normalization and assessment of (pre-)analytical variables

Autor: Edward Geeurickx, Olivier De Wever, Bruno G. De Geest, Pekka Rappu, Lien Lippens, An Hendrix

Publikováno v: Nature protocols. 16(2)

The diagnostic and therapeutic use of extracellular vesicles (EV) is under intense investigation and may lead to societal benefits. Reference materials are an invaluable resource for developing, improving and assessing the performance of regulated EV

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93e2704902f61d89adbbc0a24d4d7187
https://pubmed.ncbi.nlm.nih.gov/33452501

Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine

Autor: Piet Ost, Bert Dhondt, Nicolaas Lumen, Edward Geeurickx, An Hendrix, Joeri Tulkens, Jyrki Heino, Glenn Vergauwen, Olivier De Wever, Jan Van Deun, Lien Lippens, Pekka Rappu, Ilkka Miinalainen

Publikováno v: JOURNAL OF EXTRACELLULAR VESICLES
Journal of Extracellular Vesicles, Vol 9, Iss 1 (2020)
Journal of Extracellular Vesicles

Extracellular vesicles (EV) are increasingly being recognized as important vehicles of intercellular communication and promising diagnostic and prognostic biomarkers in cancer. Despite this enormous clinical potential, the plethora of methods to sepa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec9a04dea5cf971c5d8cfabafe911bc3
https://biblio.ugent.be/publication/8653373

The generation and use of recombinant extracellular vesicles as biological reference material

Autor: Nico Callewaert, Katarzyna Bloch, Esther N. M. Nolte-‘t-Hoen, Ilkka Miinalainen, Thomas De Beer, Elisa Heyrman, Bert Dhondt, Edward Geeurickx, Francis Impens, Hannelore Denys, Edwin van der Pol, Lien Lippens, Jan Van Deun, Glenn Vergauwen, Johannes V. Swinnen, Sven Eyckerman, Geert Braems, Rienk Nieuwland, Pieter-Jan Van Bockstal, Joeri Tulkens, Olivier De Wever, Pieter Mestdagh, Kris Gevaert, Delphine De Sutter, An Hendrix, Marca H. M. Wauben, Jo Vandesompele

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Nature Communications, 10(1), 1. Nature Publishing Group
Nature Communications
Nature communications, 10(1):3288. Nature Publishing Group
NATURE COMMUNICATIONS

Recent years have seen an increase of extracellular vesicle (EV) research geared towards biological understanding, diagnostics and therapy. However, EV data interpretation remains challenging owing to complexity of biofluids and technical variation i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::313fbc62b1e790dd0d7d39a560a36719
https://dspace.library.uu.nl/handle/1874/390844