Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Edward C. Heath"'
Autor:
Edward C. Heath, Kathleen A. Presper
Publikováno v:
Archives of Biochemistry and Biophysics. 246:460-468
Iowa trypanosome antigen type (IaTat) 1.2 variant surface glycoprotein (VSG) is synthesized in vitro as a Mr 54,000 preprotein that contains a 31-amino-acid signal peptide. Translation of mRNA in the presence of either dog pancreas or trypanosome mic
Autor:
Edward C. Heath, Joan M. Hanley
Publikováno v:
Archives of Biochemistry and Biophysics. 239:404-419
The heterotetrameric plasma glycoprotein rat haptoglobin previously was shown to be synthesized by hepatocytes in a precursor form, prohaptoglobin, which contains one α-subunit region and one β-subunit region. Two of these molecules, each with a mo
Autor:
Gary L. Firestone, Edward C. Heath
Publikováno v:
Archives of Biochemistry and Biophysics. 201:453-467
The effects of dibutyryl cyclic AMP on glycoprotein biosynthesis, intracellular mobilization, and secretion in isolated rat hepatocytes are described. Dibutyryl cyclic AMP (2.5 m m ) initially suppresses [ 3 H]glucosamine or [ 3 H]fucose incorporatio
Autor:
Edward C. Heath, Susan T. Brownlee
Publikováno v:
Archives of Biochemistry and Biophysics. 166:1-7
A 5′-nucleotidase which has both monoesterase and diesterase activity has been isolated from the growth medium of Micrococcus sodonensis. The enzyme can be separated electrophoretically into three isoenzymes. The enzyme exhibited a pH optimum of 8.
Autor:
Edward C. Heath, James A. Braatz
Publikováno v:
Journal of Biological Chemistry. 249:2536-2547
Cell suspensions of Micrococcus sodonensis (ATCC 11880) secrete 7 to 10 individual proteins including an alkaline phosphatase, a nuclease, and a protease. The appearance of enzyme activities in the extracellular medium is dependent on the co-secretio
Autor:
Edward C. Heath, S. J. Turco
Publikováno v:
Journal of Biological Chemistry. 252:2918-2928
Incubation of SV40-transformed human lung fibroblasts with [3H]glucosamine for 1 h. followed by chloroform:methanol extraction and thin layer chromatographic analysis, revealed the presence of a major radioactive lipid that was isolated and character
Autor:
Nancy J. Stark, Edward C. Heath
Publikováno v:
Archives of Biochemistry and Biophysics. 192:599-609
The mouse myeloma tumor, MOPC-46, produces a kappa-type immunoglobulin light chain that may be isolated from the urine of tumor-bearing animals. This protein possesses a single carbohydrate side chain, attached by glycosylamine linkage to asparagine
Autor:
Warren V. Johnson, Edward C. Heath
Publikováno v:
Archives of Biochemistry and Biophysics. 251:732-737
Native bovine fetuin is a major α1-glycoprotein in the serum and cerebrospinal fluid of fetal calves. We previously reported (Johnson, W. V., and Heath, E. C. (1986) Biochemistry25, 5518–5525) the purification of the primary translation product fo
Autor:
Kathryn M. Verbanac, Edward C. Heath
Publikováno v:
Archives of Biochemistry and Biophysics. 223:149-157
Various biosynthetic forms of rat alpha 1-antitrypsin (alpha 1AT) have been isolated by immunoprecipitation of in vitro and in vivo synthesized products. Rat alpha 1AT is synthesized in a rabbit reticulocyte system as a 45,000-Da preprotein with a 23
Publikováno v:
Journal of Biological Chemistry. 246:5596-5606
A capsular polysaccharide produced by Aerobacter aerogenes has been purified and shown to consist of galactose, mannose, and glucuronic acid in a molar ratio of 2:1:1, respectively. Partial acid hydrolysis liberated an aldobiouronic acid identified a