Maximizing microbial bioproduction from sustainable carbon sources using iterative systems engineering

Autor: Thomas Eng, Deepanwita Banerjee, Javier Menasalvas, Yan Chen, Jennifer Gin, Hemant Choudhary, Edward Baidoo, Jian Hua Chen, Axel Ekman, Ramu Kakumanu, Yuzhong Liu Diercks, Alex Codik, Carolyn Larabell, John Gladden, Blake A. Simmons, Jay D. Keasling, Christopher J. Petzold, Aindrila Mukhopadhyay

Publikováno v: Cell Reports, Vol 42, Iss 9, Pp 113087- (2023)

Summary: Maximizing the production of heterologous biomolecules is a complex problem that can be addressed with a systems-level understanding of cellular metabolism and regulation. Specifically, growth-coupling approaches can increase product titers

Externí odkaz: https://doaj.org/article/44231f8beb664554bcb8285ca80eab71

Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts

Autor: Junko Yaegashi, James Kirby, Masakazu Ito, Jian Sun, Tanmoy Dutta, Mona Mirsiaghi, Eric R. Sundstrom, Alberto Rodriguez, Edward Baidoo, Deepti Tanjore, Todd Pray, Kenneth Sale, Seema Singh, Jay D. Keasling, Blake A. Simmons, Steven W. Singer, Jon K. Magnuson, Adam P. Arkin, Jeffrey M. Skerker, John M. Gladden

Publikováno v: Biotechnology for Biofuels, Vol 10, Iss 1, Pp 1-13 (2017)

Abstract Background Economical conversion of lignocellulosic biomass into biofuels and bioproducts is central to the establishment of a robust bioeconomy. This requires a conversion host that is able to both efficiently assimilate the major lignocell

Externí odkaz: https://doaj.org/article/8c4c6cf548ee4189b653303f07a002f6

Leveraging propionate-induced growth inhibition in Corynebacterium glutamicum to evolve improved methylmalonyl-CoA-dependent polyketide production

Autor: Jay Keasling, chunjun zhan, Namil Lee, Guangxu Lan, Qingyun Dan, Aidan Cowan, Zilong Wang, Edward Baidoo, Ramu Kakumanu, Bridget Luckie, Rita Kuo, Joshua McCauley, Robert Haushalter

Corynebacterium glutamicum is a promising host for production of valuable polyketides. Propionate addition, a strategy known to increase polyketide production by increasing intracellular methylmalonyl-CoA availability, causes growth inhibition in C.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::367065113a4315734dbe80c96c2638de
https://doi.org/10.21203/rs.3.rs-2200679/v1

A microbial supply chain for production of the anti-cancer drug vinblastine

Autor: Jie Zhang, Lea G. Hansen, Olga Gudich, Konrad Viehrig, Lærke M. M. Lassen, Lars Schrübbers, Khem B. Adhikari, Paulina Rubaszka, Elena Carrasquer-Alvarez, Ling Chen, Vasil D’Ambrosio, Beata Lehka, Ahmad K. Haidar, Saranya Nallapareddy, Konstantina Giannakou, Marcos Laloux, Dushica Arsovska, Marcus A. K. Jørgensen, Leanne Jade G. Chan, Mette Kristensen, Hanne B. Christensen, Suresh Sudarsan, Emily A. Stander, Edward Baidoo, Christopher J. Petzold, Tune Wulff, Sarah E. O’Connor, Vincent Courdavault, Michael K. Jensen, Jay D. Keasling

Publikováno v: Nature
Zhang, J, Hansen, L G, Gudich, O, Viehrig, K, Lassen, L M M, Schrübbers, L, Adhikari, K B, Rubaszka, P, Carrasquer-Alvarez, E, Chen, L, D’Ambrosio, V, Lehka, B, Haidar, A K, Nallapareddy, S, Giannakou, K, Laloux, M, Arsovska, D, Jørgensen, M A K, Chan, L J G, Kristensen, M, Christensen, H B, Sudarsan, S, Stander, E A, Baidoo, E, Petzold, C J, Wulff, T, O’Connor, S E, Courdavault, V, Jensen, M K & Keasling, J D 2022, ' A microbial supply chain for production of the anti-cancer drug vinblastine ', Nature, vol. 609, no. 7926, pp. 341-347 . https://doi.org/10.1038/s41586-022-05157-3
Nature, vol 609, iss 7926

Monoterpene indole alkaloids (MIAs) are a diverse family of complex plant secondary metabolites with many medicinal properties, including the essential anti-cancer therapeutics vinblastine and vincristine1. As MIAs are difficult to chemically synthes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19bacac052957ae55ba7af120ea83ec4
https://pubmed.ncbi.nlm.nih.gov/36370691

Metabolic engineering for the high-yield production of isoprenoid-based C₅ alcohols in E. coli

Autor: Kevin W, George, Mitchell G, Thompson, Aram, Kang, Edward, Baidoo, George, Wang, Leanne Jade G, Chan, Paul D, Adams, Christopher J, Petzold, Jay D, Keasling, Taek Soon, Lee

Publikováno v: Scientific Reports

Branched five carbon (C5) alcohols are attractive targets for microbial production due to their desirable fuel properties and importance as platform chemicals. In this study, we engineered a heterologous isoprenoid pathway in E. coli for the high-yie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fa1ab552a6eb033bc19d8d1482c9b970
https://pubmed.ncbi.nlm.nih.gov/26052683