Role of Fasciola hepatica Small RNAs in the Interaction With the Mammalian Host

Autor: Santiago Fontenla, Mauricio Langleib, Eduardo de la Torre-Escudero, Maria Fernanda Domínguez, Mark W. Robinson, José Tort

Publikováno v: Frontiers in Cellular and Infection Microbiology, Vol 11 (2022)

MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression being involved in many different biological processes and play a key role in developmental timing. Additionally, recent studies have shown that miRNAs released from p

Externí odkaz: https://doaj.org/article/fbc747ea5b904ccb9370ffe87f2b15f8

Fasciola hepatica Gastrodermal Cells Selectively Release Extracellular Vesicles via a Novel Atypical Secretory Mechanism

Autor: Adam Bennett, Eduardo de la Torre-Escudero, Susan Dermott, Lawrence Threadgold, Robert Hanna, Mark Robinson

Publikováno v: International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5525
Bennett, A P S, de la Torre-Escudero, E, Dermott, S S E, Threadgold, L T, Hanna, R E B & Robinson, M W 2022, ' Fasciola hepatica gastrodermal cells selectively release extracellular vesicles via a novel atypical secretory mechanism ', International Journal of Molecular Sciences, vol. 23, no. 10, 5525 . https://doi.org/10.3390/ijms23105525

The liver fluke, Fasciola hepatica, is an obligate blood-feeder, and the gastrodermal cells of the parasite form the interface with the host’s blood. Despite their importance in the host–parasite interaction, in-depth proteomic analysis of the ga

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cf087ac90b04d87a7108e9882c0e404

Helminth genome analysis reveals conservation of extracellular vesicle biogenesis pathways but divergence of RNA loading machinery between phyla

Autor: Mark W. Robinson, Adam P.S. Bennett, Eduardo de la Torre-Escudero

Publikováno v: Bennett, A, de la Torre Escudero, E & Robinson, M 2020, ' Helminth genome analysis reveals conservation of extracellular vesicle biogenesis pathways but divergence of RNA loading machinery between phyla. ', International Journal for Parasitology . https://doi.org/10.1016/j.ijpara.2020.04.004

A comparative genomics approach was used to determine whether established extracellular vesicle biogenesis pathways are conserved in helminths. This revealed conservation of membrane and cytoskeletal organising proteins as well as the endosomal sorti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ff926fb1b5d2dbef3af8b097a29bc51
https://pure.qub.ac.uk/en/publications/helminth-genome-analysis-reveals-conservation-of-extracellular-vesicle-biogenesis-pathways-but-divergence-of-rna-loading-machinery-between-phyla(06a0806b-9028-4130-9c7f-4c4e8e3c21ea).html

Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

Autor: Eduardo, de la Torre-Escudero, Mark W, Robinson

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2137

Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c608adbf4cb080f7e724798ddeeb722b
https://pubmed.ncbi.nlm.nih.gov/32399919

Surface molecules of extracellular vesicles secreted by the helminth pathogen Fasciola hepatica direct their internalisation by host cells

Autor: Eduardo, de la Torre-Escudero, Jared Q, Gerlach, Adam P S, Bennett, Krystyna, Cwiklinski, Heather L, Jewhurst, Kathryn M, Huson, Lokesh, Joshi, Michelle, Kilcoyne, Sandra, O'Neill, John P, Dalton, Mark W, Robinson

Publikováno v: PLoS Neglected Tropical Diseases

Helminth parasites secrete extracellular vesicles (EVs) that can be internalised by host immune cells resulting in modulation of host immunity. While the molecular cargo of EVs have been characterised in many parasites, little is known about the surf

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::aa541ab8bcbb88f49a21ac2097b9ccdd
https://pubmed.ncbi.nlm.nih.gov/30657764