Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Eduardo Alzola"'
Publikováno v:
British Journal of Pharmacology. 129:703-708
The permeabilizing effect of P2X(7) agonists was tested in rat submandibular acinar cells using the uptake of ethidium bromide as an index. The uptake of ethidium bromide by acini incubated at 37 degrees C in the presence of 1 mM ATP increased with t
Autor:
Mourad Metioui, Y Rodrig, Eduardo Alzola, Jean-Paul Dehaye, M Fernández, Arie Moran, David J. Fogarty, Laurence Lagneaux, Carlos Matute, Aida Marino
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1439:395-405
Epithelial cells were isolated from rat trachea by incubation of the organ in a calcium-free medium. The intracellular concentration of calcium ([Ca(2+)](i)) was measured with the calcium-sensitive fluorescent dye fura2. In resting conditions, the ce
Autor:
Jean-Paul Dehaye, Elie Kabre, Carlos Matute, Arantza Pérez-Etxebarria, Mourad Metioui, Eduardo Alzola, Jose M. Macarulla, Aida Marino, Naima Chaib, David J. Fogarty
Publikováno v:
Journal of Biological Chemistry. 273:30208-30217
Isolated ductal cells of rat submandibular gland phospholipid pools were labeled with [3H]arachidonic acid (AA). The tracer was incorporated preferentially to phosphatidylcholine (46% of the lipidic fraction). Extracellular ATP induced the release of
Autor:
A Elyamani, Eduardo Alzola, Arie Moran, Jean-Paul Dehaye, Mourad Metioui, Naima Chaib, Aida Marino, Hagai Amsallem
Publikováno v:
Journal of Cellular Physiology. 168:462-475
The effect of extracellular ATP on the intracellular calcium concentration ([Ca2+]i) in rat submandibular glands was tested. The dose-response curve for ATP was biphasic with a first increase in the 1-30 microM concentration range and a further incre
Publikováno v:
Cellular signalling. 13(7)
We have compared the formation of pores in rat submandibular acinar cells in response to 2',3'-O-(4-benzoylbenzoyl) adenosine 5'-triphosphate (Bz-ATP) and maitotoxin. Bz-ATP (100 microM) permeabilized the cells to ethidium bromide. The uptake of ethi
Autor:
Jean-Paul Dehaye, Elie Kabre, Eduardo Alzola, Naima Chaib, C Dantinne, Mourad Metioui, Aida Marino
Publikováno v:
Cell calcium. 23(6)
The intracellular concentration of calcium ([Ca2+]i) of rat submandibular ductal cells was measured with the intracellular fluorescent dye Fura-2. Carbachol (100 microM) and ATP (1 mM) both increased the [Ca2+]i. The late response to ATP was blocked
Autor:
Jean-Paul Dehaye, Aida Marino, Naima Chaib, Elie Kabre, Arie Moran, Eduardo Alzola, Hagai Amsallem, Mourad Metioui
Publikováno v:
Peptides. 19(10)
A cellular suspension from rat submandibular glands was prepared with collagenase. The intracellular pH (pHi) was estimated with 2',7'-bis-(2-carboxy-ethyl)-5(6)-carboxyfluorescein (BCECF). After exposure to NH4Cl, the pHi transiently increased (diff
Study of nonspecific cation channel coupled to P2z purinergic receptors using an acid load technique
Autor:
Jean-Paul Dehaye, Mourad Metioui, Eduardo Alzola, M Lachish, Elie Kabre, Arie Moran, Naima Chaib, Aida Marino, Katrina Grosfils
Publikováno v:
The American journal of physiology. 271(6 Pt 1)
The intracellular pH (pHi) of rat submandibular cells was measured by 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). The cells recovered from ammonium (30 mM) prepulse to their resting pHi within 10 min. Ethylisopropylamiloride (EIPA), an i