Cloning, preparation and characterization of biologically active recombinant caprine placental lactogen

Autor: C Bignon, Edna Sakal, Arieh Gertler, Jean Djiane, N Daniel, J Paly, N Chapnik-Cohen, L Belair

Publikováno v: Journal of Endocrinology
Journal of Endocrinology, BioScientifica, 1998, 159 (3), pp.509-518. ⟨10.1677/joe.0.1590509⟩
Journal of Endocrinology 3 (159), 509-518. (1998)

International audience; Caprine placental lactogen (cPL) cDNA was cloned by reverse transcription (RT)-PCR from total RNA of goat placenta. The PCR product encoding for the mature protein was gel purified, ligated to pGEM-T and finally subcloned into

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::732888ce4994543e4fa52c89be2a5e82
https://doi.org/10.1677/joe.0.1590509

Recombinant Carp (Cyprinus carpio) Growth Hormone: Expression, Purification, and Determination of Biological Activity in Vitro and in Vivo

Autor: Edna Sakal, Avigdor Levanon, Violet Daniel, Orli Lipshitz, Arieh Gertler, Dorit Vashdi, Mira Fine

Publikováno v: General and Comparative Endocrinology. 89:51-61

Carp growth hormone (cGH) cDNA (Koren et al., 1989) was cloned under the control of lambda-phage PLOL promoter and lambda cll ribosomal binding site into pBR322 plasmid to enable its expression in Escherichia coli A1645 that produces constitutively t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::088427423a0d6b988430f9d10f13737a
https://doi.org/10.1006/gcen.1993.1008

Detection and determination ofLocusta migratoria trypsin by radioimmunoassay

Autor: Edna Sakal, Yehudith Birk, Shalom W. Applebaum

Publikováno v: Archives of Insect Biochemistry and Physiology. 20:157-164

Polyclonal antibodies were produced against trypsin-like enzyme TLE-2, one of two trypsins isolated from the midgut cecae of the locust Locusta migratoria. Using those antibodies a heterologous RIA was developed. The specificity of the antibodies was

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::444664b4b615cfe528a5c065e3fd4e46
https://doi.org/10.1002/arch.940200207

Hydroxyphenyl acetate derivatives inhibit protein tyrosine kinase activity and proliferation in Nb2 rat lymphoma cells and insulin-induced lipogenesis in rat adipocytes

Autor: Edna Sakal, Arieh Gertler, Nava Cohen-Chapnik, Ruth Cohen, Yoram Shechter

Publikováno v: Molecular and Cellular Endocrinology. 80:183-192

The ortho, meta, and para forms of hydroxyphenyl acetate were found to be inhibitory in the order of ortho > para > meta in three distinct biological assays: (a) insulin-dependent assimilation of glucose into lipids in intact adipocytes, (b) growth a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de3c44a6ef65265a08751a0ea0456342
https://doi.org/10.1016/0303-7207(91)90155-l

Selective modification of recombinant bovine placental lactogen by site-directed mutagenesis at its C terminus

Autor: Gwen G. Krivi, Edna Sakal, N.R. Staten, Arieh Gertler, Jean Djiane, Russell E. McKinnie, Dorit Vashdi-Elberg

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1996, 271 (10), pp.5558-5564. ⟨10.1074/jbc.271.10.5558⟩
Journal of Biological Chemistry 10 (271), 5558-5564. (1996)

Five recombinant analogues of bovine placental lactogen (bPL) ((bPL(S184H), bPL(S187A), bPL(S187F), bPL(T188F), bPL(T188F,I190F)) were prepared, expressed in Escherichia coli, and purified to homogeneity. Circular dichroism analysis revealed no or mi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be53e930dae3b51d98a9411dd5133b78
https://hal.inrae.fr/hal-02687643