Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Edgardo Elvio Guibert"'
Publikováno v:
BioTechniques, Vol 50, Iss 4, Pp 251-254 (2011)
An inexpensive modular perfused chamber (MPC) designed for low- and normal-temperature live-cell imaging is presented. The device consists of four lathed pieces of stainless steel assembled as a cylindrical open chamber that can hold either round or
Externí odkaz:
https://doaj.org/article/7959b939db47461d9a4a593619b19412
Publikováno v:
Annals of Hepatology, Vol 1, Iss 3, Pp 140-146 (2002)
We examined how different media composition of rewarming solutions affected ammonium detoxification function, urea synthesis and the viability of hepatocytes after 72 hs of cold storage in UW solution. Freshly isolated rat hepatocytes were incubated
Externí odkaz:
https://doaj.org/article/eaddfa02afb84ea2b2cbc80f4cb9d9f4
Autor:
Edgardo Elvio Guibert, María Gabriela Mediavilla, María Eugenia Mamprin, Joaquín Valentín Rodríguez
Publikováno v:
Cell Transplantation, Vol 7 (1998)
High yields of intact parenchymal cells are produced by the two-step Digitonin-collagenase perfusion of whole liver, and it has gained wide acceptance for biochemical and cellular analyses of zonal hepatocytes. The development reached by this methodo
Externí odkaz:
https://doaj.org/article/e36bfcfa6aea4f758c25f5938a7e947c
Publikováno v:
Cell Transplantation, Vol 4 (1995)
The effect of different pH of resuspension media on the viability of hepatocytes preserved (for 96 h at 4°C) in University of Wisconsin solution (UW solution) was analyzed. After this cold resuspension media storage, we evaluated the rewarming step
Externí odkaz:
https://doaj.org/article/4e7e8a7502e74b5980240caaafb2a8fa
Publikováno v:
Cryobiology. 109:3
Autor:
Matías Emanuel Carnevale, Leonardo Juan de Paz, Pablo Stringa, Natalia Raquel Lausada, Mariana Alejandra Machuca, Joaquin Valentin Rodriguez, Claudio Tiribelli, Martín Rumbo, G E Gondolesi, Edgardo Elvio Guibert
Publikováno v:
Liver Transplantation. 25:1375-1386
Organ transplantation is the treatment of choice against terminal and irreversible organ failure. Optimal preservation of the graft is crucial to counteract cold ischemia effects. As we developed an N,N-bis-2-hydroxyethyl-2-aminoethanesulfonic acid
Autor:
Leonardo Juan, de Paz, Maria Celeste, Robert, Daniel Adolfo, Graf, Edgardo Elvio, Guibert, Joaquin Valentin, Rodriguez
Publikováno v:
Cryo letters. 36(6)
Slow cooling is a cryopreservation methodology where samples are cooled to its storage temperature at controlled cooling rates.Design, construction and evaluation of a simple and low cost device for slow cooling of small biological samples.The device