Beta-globin LCR and intron elements cooperate and direct spatial reorganization for gene therapy.

Autor: Alla Buzina, Mandy Y M Lo, Angela Moffett, Akitsu Hotta, Eden Fussner, Rikki R Bharadwaj, Peter Pasceri, J Victor Garcia-Martinez, David P Bazett-Jones, James Ellis

Publikováno v: PLoS Genetics, Vol 4, Iss 4, p e1000051 (2008)

The Locus Control Region (LCR) requires intronic elements within beta-globin transgenes to direct high level expression at all ectopic integration sites. However, these essential intronic elements cannot be transmitted through retrovirus vectors and

Externí odkaz: https://doaj.org/article/927a29181dd94ee4b2b4d1b37f6fbd61

The pluripotency factor Nanog regulates pericentromeric heterochromatin organization in mouse embryonic stem cells

Autor: Nicholas P. Mullin, James Ellis, Ryuichi Nishinakamura, Eden Fussner, Ugljesa Djuric, Jasvinder Hayre, Calvin Tang, Clara Lopes Novo, Sarah Elderkin, Peter J. Rugg-Gunn, Ian Chambers, David P. Bazett-Jones, Kashif Ahmed, Arnold R. Sienerth, Natasha P. Morgan

Publikováno v: Lopes Novo, C, Tang, C, Ahmed, K, Djuric, U, Fussner, E, Mullin, N, Morgan, N, Hayre, J, Sienerth, A, Elderkin, S, Nishinakamura, R, Chambers, I, Ellis, J, Bazett-Jones, D P & Rugg-Gunn, P J 2016, ' The pluripotency factor Nanog regulates pericentromeric heterochromatin organization in mouse embryonic stem cells ', Genes & Development . https://doi.org/10.1101/gad.275685.115

An open and decondensed chromatin organization is a defining property of pluripotency. Several epigenetic regulators have been implicated in maintaining an open chromatin organization, but how these processes are connected to the pluripotency network

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fb010720e0da516c395170a06f17304
https://doi.org/10.1101/gad.275685.115

Constitutive heterochromatin reorganization during somatic cell reprogramming

Autor: Eden Fussner, Fredrik Lanner, David P. Bazett-Jones, James Ellis, Mike Strauss, F. Jeffrey Dilworth, Carolina Perez-Iratxeta, Akitsu Hotta, Ugljesa Djuric

Publikováno v: The EMBO Journal. 30:1778-1789

Induced pluripotent stem (iPS) cell reprogramming is a gradual epigenetic process that reactivates the pluripotent transcriptional network by erasing and establishing repressive epigenetic marks. In contrast to loci-specific epigenetic changes, heter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0d00fbc8fde6da665812bfbdcb38efef
https://doi.org/10.1038/emboj.2011.96

Genome-wide dynamics of replication timing revealed by in vitro models of mouse embryogenesis

Autor: David M. Gilbert, Stephen Dalton, Kathrin Plath, Michael Kulik, Peter D. Rathjen, Tyrone Ryba, Eden Fussner, David P. Bazett-Jones, Mari Itoh, Bernadett Papp, Ichiro Hiratani, Joy Rathjen

Publikováno v: Genome Research. 20:155-169

Differentiation of mouse embryonic stem cells (mESCs) is accompanied by changes in replication timing. To explore the relationship between replication timing and cell fate transitions, we constructed genome-wide replication-timing profiles of 22 inde

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e575546ac84656e7af897c4066760976
https://doi.org/10.1101/gr.099796.109

Phase transition of a disordered nuage protein generates environmentally responsive membraneless organelles

Autor: Timothy J, Nott, Evangelia, Petsalaki, Patrick, Farber, Dylan, Jervis, Eden, Fussner, Anne, Plochowietz, Timothy D, Craggs, David P, Bazett-Jones, Tony, Pawson, Julie D, Forman-Kay, Andrew J, Baldwin

Publikováno v: Molecular cell. 57(5)

Cells chemically isolate molecules in compartments to both facilitate and regulate their interactions. In addition to membrane-encapsulated compartments, cells can form proteinaceous and membraneless organelles, including nucleoli, Cajal and PML bodi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::dc643cadecd53cca9338883055ced47e
https://pubmed.ncbi.nlm.nih.gov/33473198