Slow-Onset, Potent Inhibition of Mandelate Racemase by 2-Formylphenylboronic Acid. An Unexpected Adduct Clasps the Catalytic Machinery.

Autor: Douglas CD; Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada., Grandinetti L; Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53201-1881, United States., Easton NM; Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada., Kuehm OP; Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada., Hayden JA; Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada., Hamilton MC; Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada., St Maurice M; Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53201-1881, United States., Bearne SL; Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada.; Department of Chemistry, Dalhousie University, Halifax, NS B3H 4R2, Canada.

Publikováno v: Biochemistry [Biochemistry] 2021 Aug 02. Date of Electronic Publication: 2021 Aug 02.

A continuous assay for l-talarate/galactarate dehydratase using circular dichroism.

Autor: Easton NM; Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada., Aboushawareb SAE; Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada., Bearne SL; Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada; Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada. Electronic address: sbearne@dal.ca.

Publikováno v: Analytical biochemistry [Anal Biochem] 2018 Mar 01; Vol. 544, pp. 80-86. Date of Electronic Publication: 2017 Dec 14.